Detailed information of TA system TA111967

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5733754..5734349
• Replicon: chromosome
• Accession: NZ_CP032552
• Organism: Pseudomonas aeruginosa strain PA34

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: PA34_RS27625
• Protein ID: WP_003113526.1
• Coordinates: 5734071..5734349 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PA34_RS27620
• Protein ID: WP_003133769.1
• Coordinates: 5733754..5734059 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA34_RS27585	5729779..5730090	+	312	WP_033988980.1	hypothetical protein	-
PA34_RS27590	5730093..5730314	-	222	WP_033988977.1	hypothetical protein	-
PA34_RS27595	5730314..5730952	-	639	WP_033988974.1	DNA-binding protein	-
PA34_RS27600	5731927..5732703	-	777	WP_033989079.1	hypothetical protein	-
PA34_RS27605	5732728..5732841	-	114	Protein_5323	RepA	-
PA34_RS27610	5732880..5733092	-	213	WP_033988972.1	AlpA family phage regulatory protein	-
PA34_RS27620	5733754..5734059	-	306	WP_003133769.1	HigA family addiction module antidote protein	Antitoxin
PA34_RS27625	5734071..5734349	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PA34_RS27635	5734678..5736906	+	2229	WP_033988971.1	TonB-dependent receptor	-
PA34_RS27640	5736976..5737623	-	648	WP_003095021.1	carbonate dehydratase	-
PA34_RS27645	5737685..5738923	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T111967 WP_003113526.1 NZ_CP032552:c5734349-5734071 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T111967 NZ_CP032552:c5734349-5734071 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT111967 WP_003133769.1 NZ_CP032552:c5734059-5733754 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT111967 NZ_CP032552:c5734059-5733754 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3