Detailed information of TA system TA111925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 681574..682139
• Replicon: chromosome
• Accession: NZ_CP032519
• Organism: Cupriavidus oxalaticus strain T2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D2917_RS15960
• Protein ID: WP_151071244.1
• Coordinates: 681768..682139 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A375GBB0
• Locus tag: D2917_RS15955
• Protein ID: WP_063236948.1
• Coordinates: 681574..681771 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D2917_RS15940	677159..678127	-	969	WP_151071242.1	formimidoylglutamase	-
D2917_RS15945	678124..679443	-	1320	WP_063236946.1	MFS transporter	-
D2917_RS15950	679641..681311	-	1671	WP_151071243.1	urocanate hydratase	-
D2917_RS15955	681574..681771	+	198	WP_063236948.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
D2917_RS15960	681768..682139	+	372	WP_151071244.1	type II toxin-antitoxin system VapC family toxin	Toxin
D2917_RS15965	682190..682906	-	717	WP_151071245.1	histidine utilization repressor	-
D2917_RS15970	682942..686331	-	3390	WP_151071246.1	diguanylate cyclase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-46)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13266.28 Da
• Isoelectric Point: 5.5095

>T111925 WP_151071244.1 NZ_CP032519:681768-682139 [Cupriavidus oxalaticus]
MILVDTSVWIDHLRTGDAALAALLDASRVLVHPFVIGEIALGSLRQRDEVLGALRDLPGAVVATDDEVDRMIDDQKLYGI
GIGYVDAHLLAATRLTPGSALWTRDRRLLAVAEHLGLAARPAH

Nucleotide

• Length: 372 bp

>T111925 NZ_CP032519:681768-682139 [Cupriavidus oxalaticus]
ATGATCCTGGTCGACACCTCGGTATGGATCGACCATCTGCGTACCGGCGACGCGGCACTAGCGGCTTTGCTCGACGCCAG
CCGGGTCCTGGTGCATCCGTTTGTGATCGGCGAGATCGCGCTGGGTAGCCTGCGGCAGCGGGACGAGGTGCTGGGCGCTT
TGCGTGATTTGCCAGGCGCCGTCGTGGCGACGGACGACGAAGTCGACCGGATGATCGATGACCAGAAGTTGTACGGGATA
GGGATTGGCTACGTGGATGCGCATCTGCTGGCCGCCACCCGATTGACGCCCGGCAGTGCGCTATGGACGCGGGATCGTCG
CCTGCTCGCGGTCGCCGAGCATTTGGGGCTGGCCGCGCGGCCTGCGCATTAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7215.22 Da
• Isoelectric Point: 10.6625

>AT111925 WP_063236948.1 NZ_CP032519:681574-681771 [Cupriavidus oxalaticus]
MRTTLALDDDLLRKAQALTGLSEKSSLVREALRALIERESARRLSRLGGTEPDLESAPRRRAEPA

Nucleotide

• Length: 198 bp

>AT111925 NZ_CP032519:681574-681771 [Cupriavidus oxalaticus]
ATGCGCACCACGCTTGCCCTGGACGATGATTTGCTGCGCAAGGCCCAGGCCCTGACCGGCCTGTCCGAGAAATCCTCGCT
CGTGCGCGAAGCGCTGCGGGCACTGATCGAGCGCGAAAGCGCGCGCCGCCTGAGCCGTCTCGGCGGTACCGAACCCGACC
TGGAATCCGCGCCGCGCCGTCGCGCGGAGCCGGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A375GBB0