Detailed information of TA system TA111867

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 690868..691396
• Replicon: chromosome
• Accession: NZ_CP032513
• Organism: Shigella flexneri strain SFL1520

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: D6T62_RS03570
• Protein ID: WP_236743520.1
• Coordinates: 691166..691396 (-)
• Length: 77 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q32DW6
• Locus tag: D6T62_RS03565
• Protein ID: WP_000147081.1
• Coordinates: 690868..691176 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D6T62_RS03540 (686292)	686292..687505	+	1214	WP_167701210.1	IS3 family transposase	-
D6T62_RS03545 (687545)	687545..689893	+	2349	Protein_683	toprim domain-containing protein	-
D6T62_RS03550 (689968)	689968..690246	+	279	WP_005067014.1	hypothetical protein	-
D6T62_RS03555 (690243)	690243..690653	+	411	WP_000814621.1	recombination protein NinB	-
D6T62_RS03560 (690650)	690650..690826	+	177	WP_001254220.1	NinE family protein	-
D6T62_RS03565 (690868)	690868..691176	-	309	WP_000147081.1	helix-turn-helix transcriptional regulator	Antitoxin
D6T62_RS03570 (691166)	691166..691396	-	231	WP_236743520.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D6T62_RS03575 (691409)	691409..692377	+	969	WP_000961310.1	Rha family phage regulatory protein	-
D6T62_RS03580 (692374)	692374..692607	+	234	WP_000618004.1	hypothetical protein	-
D6T62_RS03585 (692594)	692594..693501	+	908	Protein_691	DNA-binding protein	-
D6T62_RS03590 (693512)	693512..694390	+	879	WP_000988187.1	ATP-binding protein	-
D6T62_RS03595 (694387)	694387..695634	+	1248	Protein_693	replicative DNA helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ipaH4.5	674341..739839	65498

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (5-70)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-97)

Sequences

Toxin

Protein

• Length: 77 a.a.
• Molecular weight: 8695.15 Da
• Isoelectric Point: 10.5308

>T111867 WP_236743520.1 NZ_CP032513:c691396-691166 [Shigella flexneri]
MKVYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKTQKTPPAERKKAETRMKEVKHDW

Nucleotide

• Length: 231 bp

>T111867 NZ_CP032513:c691396-691166 [Shigella flexneri]
ATGAAGGTATACGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTTTGGTGACGGGCTTTTCGAACTCAGAATTAAAGG
CAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAGGGAAACGCATCATCATGCTGCATAGTTTTGTAAAGAAAA
CGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATGAAGGAGGTTAAGCATGACTGGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11185.94 Da
• Isoelectric Point: 8.5840

>AT111867 WP_000147081.1 NZ_CP032513:c691176-690868 [Shigella flexneri]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV

Nucleotide

• Length: 309 bp

>AT111867 NZ_CP032513:c691176-690868 [Shigella flexneri]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACACTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A4P7TJC1