Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1952410..1952912 Replicon chromosome
Accession NZ_CP032426
Organism Escherichia coli strain SCEC020001

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C7V21_RS10560 Protein ID WP_000767829.1
Coordinates 1952658..1952912 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C7V21_RS10555 Protein ID WP_001259255.1
Coordinates 1952410..1952661 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7V21_RS10525 1947587..1948654 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
C7V21_RS10530 1948654..1949724 - 1071 WP_053908449.1 histidinol-phosphate transaminase -
C7V21_RS10535 1949721..1951025 - 1305 WP_053908451.1 histidinol dehydrogenase -
C7V21_RS10540 1951031..1951930 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
C7V21_RS10550 1952076..1952126 - 51 WP_001364200.1 his operon leader peptide -
C7V21_RS10555 1952410..1952661 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C7V21_RS10560 1952658..1952912 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C7V21_RS10565 1952995..1953819 + 825 WP_053908453.1 SDR family oxidoreductase -
C7V21_RS10570 1953865..1954794 + 930 WP_053908455.1 LysR family transcriptional regulator -
C7V21_RS10575 1955009..1955071 + 63 WP_010723108.1 membrane protein YoeI -
C7V21_RS10580 1955061..1956419 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C7V21_RS10585 1956598..1957656 + 1059 WP_053908457.1 transport protein YeeE -
C7V21_RS10590 1957670..1957897 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T111781 WP_000767829.1 NZ_CP032426:1952658-1952912 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T111781 NZ_CP032426:1952658-1952912 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT111781 WP_001259255.1 NZ_CP032426:1952410-1952661 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT111781 NZ_CP032426:1952410-1952661 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References