Detailed information of TA system TA111763

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ygfYX/Cpta(toxin)
• Location: 4165920..4166562
• Replicon: chromosome
• Accession: NZ_CP032415
• Organism: Shewanella algae strain VGH117

Toxin (Protein)

• Gene name: ygfX
• Uniprot ID: -
• Locus tag: D3X10_RS19720
• Protein ID: WP_115389948.1
• Coordinates: 4166140..4166562 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: ygfY
• Uniprot ID: A0A7X5Z2N1
• Locus tag: D3X10_RS19715
• Protein ID: WP_028779782.1
• Coordinates: 4165920..4166159 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3X10_RS19690 (D3X10_19750)	4161604..4162431	+	828	WP_044734948.1	prolipoprotein diacylglyceryl transferase	-
D3X10_RS19695 (D3X10_19755)	4162431..4163225	+	795	WP_044734947.1	thymidylate synthase	-
D3X10_RS19700 (D3X10_19760)	4163342..4164516	+	1175	Protein_3661	Na+/H+ antiporter NhaA	-
D3X10_RS19705 (D3X10_19765)	4164513..4164896	+	384	WP_062793820.1	hypothetical protein	-
D3X10_RS19710 (D3X10_19770)	4164913..4165821	+	909	WP_115389947.1	transcriptional activator NhaR	-
D3X10_RS19715 (D3X10_19775)	4165920..4166159	+	240	WP_028779782.1	succinate dehydrogenase assembly factor 2	Antitoxin
D3X10_RS19720 (D3X10_19780)	4166140..4166562	+	423	WP_115389948.1	protein YgfX	Toxin
D3X10_RS19725 (D3X10_19785)	4166579..4168189	-	1611	WP_115389949.1	L-aspartate oxidase	-
D3X10_RS19730 (D3X10_19790)	4168388..4168966	+	579	WP_025010551.1	RNA polymerase sigma factor RpoE	-
D3X10_RS19735 (D3X10_19795)	4168991..4169611	+	621	WP_025887842.1	RseA family anti-sigma factor	-
D3X10_RS19740 (D3X10_19800)	4169621..4170553	+	933	WP_025887844.1	MucB/RseB C-terminal domain-containing protein	-
D3X10_RS19745 (D3X10_19805)	4170564..4171043	+	480	WP_117199820.1	SoxR reducing system RseC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (5-135)

Antitoxin

• Flavinator of succinate dehydrogenase (5-75)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 16318.83 Da
• Isoelectric Point: 7.6045

>T111763 WP_115389948.1 NZ_CP032415:4166140-4166562 [Shewanella algae]
VAGQRHRFQLSSSFSQYLSLTVLAAICLSSFLAWPALDYPFYLSFKYLCLLLVLVAFGLALWRLRRWQLCFWLNELGEGQ
FEPGSSFELSGKPWVSPFVVTFTYQGEDNYGRCWLFADMFDDTDYRHLCRLLLQRSKSAN

Nucleotide

• Length: 423 bp

>T111763 NZ_CP032415:4166140-4166562 [Shewanella algae]
GTGGCCGGGCAGCGCCATAGGTTTCAACTCAGTTCTTCCTTCAGCCAGTATCTGAGTCTGACGGTGCTGGCTGCTATTTG
CCTGAGCAGCTTTCTTGCCTGGCCGGCGCTGGATTATCCTTTCTATTTATCGTTCAAATACTTATGTTTGCTGCTGGTAC
TGGTAGCTTTTGGCCTGGCTCTGTGGCGTTTGCGCCGTTGGCAACTCTGTTTCTGGCTTAATGAATTGGGTGAAGGACAG
TTCGAACCCGGTTCGAGTTTCGAACTTAGTGGCAAACCTTGGGTCAGCCCCTTTGTGGTGACTTTTACCTATCAGGGGGA
GGATAACTACGGGCGTTGCTGGCTGTTTGCCGATATGTTTGACGACACAGACTATCGTCATCTGTGTCGTCTATTGTTGC
AGCGGTCAAAGTCCGCTAACTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9247.74 Da
• Isoelectric Point: 5.0633

>AT111763 WP_028779782.1 NZ_CP032415:4165920-4166159 [Shewanella algae]
MSTPRVKWACRRGMLELDVLFQPFVEQHYETLSPEDKALFVRLLECEDPELFAWFMGHEQCPDPQLAQMVVRVRGRAAP

Nucleotide

• Length: 240 bp

>AT111763 NZ_CP032415:4165920-4166159 [Shewanella algae]
ATGAGTACCCCCAGGGTAAAGTGGGCATGTCGCCGTGGCATGCTGGAGCTTGATGTATTGTTTCAACCTTTTGTGGAGCA
GCATTATGAGACTCTGAGCCCTGAGGACAAGGCCTTGTTTGTTCGTCTGCTTGAGTGTGAAGACCCGGAACTGTTTGCCT
GGTTTATGGGGCATGAGCAATGTCCGGATCCGCAACTGGCACAGATGGTAGTGCGCGTTCGTGGCCGGGCAGCGCCATAG

Structures

Antitoxin

• AlphaFold DB: A0A7X5Z2N1