Detailed information of TA system TA111756

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 39344..40023
• Replicon: plasmid p1
• Accession: NZ_CP032406
• Organism: Rhizobium rosettiformans strain MAE2-X

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D4A92_RS21760
• Protein ID: WP_203020549.1
• Coordinates: 39344..39775 (-)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: D4A92_RS21765
• Protein ID: WP_203020551.1
• Coordinates: 39772..40023 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D4A92_RS21740	35284..36102	-	819	WP_203020543.1	ABC transporter permease	-
D4A92_RS21745	36099..37043	-	945	WP_203020545.1	ABC transporter permease	-
D4A92_RS21750	37221..38126	-	906	WP_203020547.1	LysR family transcriptional regulator	-
D4A92_RS21755	38651..39303	+	653	Protein_32	Fic family protein	-
D4A92_RS21760	39344..39775	-	432	WP_203020549.1	type II toxin-antitoxin system VapC family toxin	Toxin
D4A92_RS21765	39772..40023	-	252	WP_203020551.1	plasmid stabilization protein	Antitoxin
D4A92_RS21770	40711..41172	+	462	WP_203020554.1	mobile mystery protein A	-
D4A92_RS21775	41169..41764	+	596	Protein_36	mobile mystery protein B	-
D4A92_RS21780	42105..42782	-	678	WP_203020556.1	class I SAM-dependent methyltransferase	-
D4A92_RS21785	42892..43356	+	465	WP_203020558.1	Rrf2 family transcriptional regulator	-
D4A92_RS21790	43435..44166	-	732	WP_203020560.1	SDR family oxidoreductase	-
D4A92_RS21795	44216..44764	-	549	WP_203020562.1	flavin reductase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG	1..510647	510647

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-126)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15544.07 Da
• Isoelectric Point: 7.0158

>T111756 WP_203020549.1 NZ_CP032406:c39775-39344 [Rhizobium rosettiformans]
MILIDTNVISEPWKPAPAATVVAWLDAQAIETLFISAISVAELRFGIASMPIGRRQTILRNRLEDEVLPHFVERILPFTL
STSRLYSDLMAGARVSGKAIGKADGLIAATAAERGLVVATRDTSPFKAAGLKIINPWIDQNFD

Nucleotide

• Length: 432 bp

>T111756 NZ_CP032406:c39775-39344 [Rhizobium rosettiformans]
ATGATCTTGATAGACACCAACGTGATATCGGAGCCGTGGAAGCCTGCTCCAGCTGCAACGGTTGTGGCTTGGTTGGATGC
CCAGGCAATCGAAACGCTCTTTATCTCTGCGATATCGGTTGCGGAGCTTCGGTTTGGCATCGCATCAATGCCTATCGGGC
GCCGTCAAACAATCCTCCGCAACCGTCTTGAGGATGAGGTCCTACCACATTTTGTTGAGCGTATTTTGCCCTTCACCCTA
AGCACCTCGCGGCTCTACTCTGATCTGATGGCAGGCGCGCGAGTATCCGGTAAAGCGATCGGCAAAGCCGATGGATTGAT
TGCGGCCACAGCTGCAGAGAGGGGGCTCGTTGTGGCAACGAGAGACACAAGCCCGTTCAAGGCAGCTGGATTGAAAATTA
TCAATCCGTGGATTGATCAGAACTTCGACTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9246.32 Da
• Isoelectric Point: 4.8616

>AT111756 WP_203020551.1 NZ_CP032406:c40023-39772 [Rhizobium rosettiformans]
MPAVTIRNLSDETHRALRVRAAHHGRSTEAEIRDILEAAVRPSQRIKLGSLLVDIGREADLSDSDVEALQERDNTPAEPM
TFE

Nucleotide

• Length: 252 bp

>AT111756 NZ_CP032406:c40023-39772 [Rhizobium rosettiformans]
ATGCCGGCAGTTACCATTCGGAATCTTTCTGATGAAACCCACCGCGCTTTGCGTGTGAGAGCAGCTCATCATGGCCGGAG
CACTGAGGCGGAAATTCGCGATATTCTTGAGGCCGCTGTTCGCCCGTCTCAACGCATAAAGCTCGGGTCATTGCTTGTGG
ACATTGGTCGGGAGGCCGACCTGTCAGACAGTGATGTTGAAGCTCTGCAAGAGCGCGACAATACGCCAGCTGAACCGATG
ACTTTCGAATGA