Detailed information of TA system TA111745

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2370887..2371587
• Replicon: chromosome
• Accession: NZ_CP032402
• Organism: Thermomonospora amylolytica strain YIM 77502

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: D3U04_RS10735
• Protein ID: WP_119731755.1
• Coordinates: 2371213..2371587 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D3U04_RS10730
• Protein ID: WP_119728067.1
• Coordinates: 2370887..2371216 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3U04_RS31730	2366938..2367090	+	153	WP_157995852.1	hypothetical protein	-
D3U04_RS10720	2367158..2370505	+	3348	WP_119728065.1	DUF4132 domain-containing protein	-
D3U04_RS10730	2370887..2371216	-	330	WP_119728067.1	helix-turn-helix domain-containing protein	Antitoxin
D3U04_RS10735	2371213..2371587	-	375	WP_119731755.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D3U04_RS10740	2371634..2371978	-	345	WP_198679456.1	HEAT repeat domain-containing protein	-
D3U04_RS10745	2372524..2375373	+	2850	WP_198679457.1	Na+/H+ antiporter subunit A	-
D3U04_RS10750	2375370..2375756	+	387	WP_119728069.1	Na(+)/H(+) antiporter subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (18-111)

Antitoxin

• HigA2-like, helix-turn-helix domain (25-99)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13962.87 Da
• Isoelectric Point: 4.6655

>T111745 WP_119731755.1 NZ_CP032402:c2371587-2371213 [Thermomonospora amylolytica]
VAWDVVLLEPVESWFLKLCESDPDTAALVEQAIDRLAEVGPTLGRPLVDTLEHSELHNLKELRPGSRGRSEIRMLFVFDP
DRAAIFLVAGDKAGQWSRWYEQAIPLAEARYAEYRAALSKEAGR

Nucleotide

• Length: 375 bp

>T111745 NZ_CP032402:c2371587-2371213 [Thermomonospora amylolytica]
GTGGCCTGGGATGTCGTCCTTCTGGAACCTGTCGAGAGCTGGTTTCTCAAGCTCTGCGAGTCCGATCCGGACACCGCAGC
GCTGGTCGAACAAGCCATCGACCGGCTTGCGGAGGTCGGGCCAACGCTGGGGCGCCCCTTGGTCGACACCCTCGAACACA
GCGAGCTGCACAACCTCAAGGAGCTCCGGCCTGGGTCACGGGGACGTTCGGAGATACGCATGTTGTTCGTCTTCGATCCG
GACCGTGCGGCCATCTTTCTTGTCGCAGGCGACAAGGCCGGTCAGTGGTCTCGGTGGTATGAACAGGCCATTCCTCTGGC
CGAGGCCCGGTATGCGGAGTACCGCGCCGCTTTGAGCAAGGAGGCAGGACGATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11844.42 Da
• Isoelectric Point: 9.7311

>AT111745 WP_119728067.1 NZ_CP032402:c2371216-2370887 [Thermomonospora amylolytica]
MSTGRNWRDIKAEAHRLHPELADPERQARARAALDAYVAGHHLKELRKSTGKTQAEVARILGVSQSRISQIENGDISAME
LETLRAYAAALGGHLDITISVGPHSVKVA

Nucleotide

• Length: 330 bp

>AT111745 NZ_CP032402:c2371216-2370887 [Thermomonospora amylolytica]
ATGAGCACTGGGCGTAACTGGCGGGACATCAAGGCCGAGGCTCATCGTCTTCACCCAGAGCTCGCCGACCCGGAGCGCCA
GGCTCGGGCACGGGCGGCACTCGACGCCTATGTCGCCGGCCACCATCTGAAGGAGCTGCGGAAGTCCACCGGCAAGACGC
AGGCCGAGGTGGCGCGCATCCTGGGCGTGTCGCAGTCCCGGATCTCCCAGATCGAGAACGGCGACATCAGTGCCATGGAA
CTGGAGACTCTCCGGGCCTATGCGGCCGCGCTCGGTGGACATCTGGACATCACCATCAGTGTCGGCCCTCACTCGGTCAA
GGTCGCCTGA