Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2373710..2374446 Replicon chromosome
Accession NZ_CP032222
Organism Klebsiella pneumoniae strain AR_0046

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag AM425_RS11800 Protein ID WP_032433360.1
Coordinates 2373710..2374192 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AM425_RS11805 Protein ID WP_003026799.1
Coordinates 2374180..2374446 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM425_RS11780 2370259..2370999 + 741 WP_050597964.1 molecular chaperone -
AM425_RS11785 2371003..2371581 + 579 WP_032432061.1 type 1 fimbrial protein -
AM425_RS11790 2371593..2372537 + 945 WP_077254249.1 fimbrial protein -
AM425_RS11795 2372750..2373349 + 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
AM425_RS11800 2373710..2374192 - 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
AM425_RS11805 2374180..2374446 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AM425_RS11810 2374759..2375322 - 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
AM425_RS11815 2375332..2375964 - 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
AM425_RS11820 2375982..2376986 - 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
AM425_RS11825 2376970..2377632 - 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
AM425_RS11830 2377661..2378800 - 1140 WP_032433368.1 mannitol dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2362767..2407278 44511


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T111411 WP_032433360.1 NZ_CP032222:c2374192-2373710 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T111411 NZ_CP032222:c2374192-2373710 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT111411 WP_003026799.1 NZ_CP032222:c2374446-2374180 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT111411 NZ_CP032222:c2374446-2374180 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References