Detailed information of TA system TA111400

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 120040..120776
• Replicon: plasmid unnamed3
• Accession: NZ_CP032209
• Organism: Klebsiella pneumoniae strain AR_0109

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: AM488_RS29460
• Protein ID: WP_003026803.1
• Coordinates: 120294..120776 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: AM488_RS29455
• Protein ID: WP_003026799.1
• Coordinates: 120040..120306 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM488_RS29410	116102..116464	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
AM488_RS29415	116514..116864	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
AM488_RS29420	117222..117491	+	270	WP_004152102.1	hypothetical protein	-
AM488_RS29425	117479..118054	+	576	WP_004152103.1	hypothetical protein	-
AM488_RS29430	118085..118579	+	495	WP_004152104.1	DNA-binding protein	-
AM488_RS29435	118623..118991	+	369	WP_004152105.1	hypothetical protein	-
AM488_RS29440	119025..119228	+	204	WP_004152106.1	hemolysin expression modulator Hha	-
AM488_RS29445	119277..119534	+	258	WP_004152107.1	hypothetical protein	-
AM488_RS29450	119610..119864	+	255	WP_004152108.1	hypothetical protein	-
AM488_RS29455	120040..120306	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
AM488_RS29460	120294..120776	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
AM488_RS29465	120988..122334	+	1347	WP_020314316.1	ISNCY family transposase	-
AM488_RS29470	122835..124089	-	1255	Protein_131	IS3 family transposase	-
AM488_RS29475	124177..125139	-	963	WP_004152113.1	M48 family metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..161618	161618

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T111400 WP_003026803.1 NZ_CP032209:120294-120776 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T111400 NZ_CP032209:120294-120776 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT111400 WP_003026799.1 NZ_CP032209:120040-120306 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT111400 NZ_CP032209:120040-120306 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66