Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 120040..120776 Replicon plasmid unnamed3
Accession NZ_CP032209
Organism Klebsiella pneumoniae strain AR_0109

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag AM488_RS29460 Protein ID WP_003026803.1
Coordinates 120294..120776 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AM488_RS29455 Protein ID WP_003026799.1
Coordinates 120040..120306 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM488_RS29410 116102..116464 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
AM488_RS29415 116514..116864 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
AM488_RS29420 117222..117491 + 270 WP_004152102.1 hypothetical protein -
AM488_RS29425 117479..118054 + 576 WP_004152103.1 hypothetical protein -
AM488_RS29430 118085..118579 + 495 WP_004152104.1 DNA-binding protein -
AM488_RS29435 118623..118991 + 369 WP_004152105.1 hypothetical protein -
AM488_RS29440 119025..119228 + 204 WP_004152106.1 hemolysin expression modulator Hha -
AM488_RS29445 119277..119534 + 258 WP_004152107.1 hypothetical protein -
AM488_RS29450 119610..119864 + 255 WP_004152108.1 hypothetical protein -
AM488_RS29455 120040..120306 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AM488_RS29460 120294..120776 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
AM488_RS29465 120988..122334 + 1347 WP_020314316.1 ISNCY family transposase -
AM488_RS29470 122835..124089 - 1255 Protein_131 IS3 family transposase -
AM488_RS29475 124177..125139 - 963 WP_004152113.1 M48 family metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..161618 161618


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T111400 WP_003026803.1 NZ_CP032209:120294-120776 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T111400 NZ_CP032209:120294-120776 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT111400 WP_003026799.1 NZ_CP032209:120040-120306 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT111400 NZ_CP032209:120040-120306 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References