Detailed information of TA system TA111398

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 269315..270225
• Replicon: plasmid unnamed2
• Accession: NZ_CP032208
• Organism: Klebsiella pneumoniae strain AR_0109

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A2N4Z2R0
• Locus tag: AM488_RS28505
• Protein ID: WP_004197882.1
• Coordinates: 269755..270225 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: AM488_RS28500
• Protein ID: WP_004181895.1
• Coordinates: 269315..269758 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM488_RS28470	264471..264686	-	216	Protein_272	IS200/IS605 family transposase	-
AM488_RS28475	264688..265257	+	570	Protein_273	transposase	-
AM488_RS28480	265625..267112	-	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
AM488_RS28490	267521..267943	+	423	WP_004197884.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
AM488_RS28495	267943..269214	+	1272	WP_004197886.1	translesion error-prone DNA polymerase V subunit UmuC	-
AM488_RS28500	269315..269758	+	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
AM488_RS28505	269755..270225	+	471	WP_004197882.1	RES family NAD+ phosphorylase	Toxin
AM488_RS28510	270335..270595	-	261	WP_004026352.1	hypothetical protein	-
AM488_RS28520	271285..272654	+	1370	WP_085949440.1	IS3-like element ISKpn8 family transposase	-
AM488_RS28525	272651..273784	-	1134	WP_119183615.1	IS481 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..310872	310872
	-	flank	IS/Tn	-	-	264871..265257	386

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17633.92 Da
• Isoelectric Point: 4.5152

>T111398 WP_004197882.1 NZ_CP032208:269755-270225 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDFFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T111398 NZ_CP032208:269755-270225 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTTTTTTACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCTCTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATTGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT111398 WP_004181895.1 NZ_CP032208:269315-269758 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT111398 NZ_CP032208:269315-269758 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A2N4Z2R0

Antitoxin

• AlphaFold DB: A0A2J4RLY9