Detailed information of TA system TA111354

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1665343..1665974
• Replicon: chromosome
• Accession: NZ_CP032204
• Organism: Escherichia coli strain AR_0013

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8T5W4M7
• Locus tag: AM340_RS08270
• Protein ID: WP_001260303.1
• Coordinates: 1665343..1665618 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: U9Y7E1
• Locus tag: AM340_RS08275
• Protein ID: WP_000593555.1
• Coordinates: 1665615..1665974 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM340_RS08255	1660347..1661414	+	1068	WP_000361489.1	HlyD family secretion protein	-
AM340_RS08260	1661411..1664146	+	2736	WP_000149108.1	ribosome-associated ATPase/putative transporter RbbA	-
AM340_RS08265	1664146..1665270	+	1125	WP_001314210.1	ABC transporter permease	-
AM340_RS08270	1665343..1665618	+	276	WP_001260303.1	type II toxin-antitoxin system HicA family toxin	Toxin
AM340_RS08275	1665615..1665974	+	360	WP_000593555.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AM340_RS08280	1666024..1666884	-	861	WP_001131789.1	class II aldolase	-
AM340_RS08285	1666916..1667185	-	270	WP_000949581.1	HPr family phosphocarrier protein	-
AM340_RS08290	1667175..1668683	-	1509	WP_012602556.1	carbohydrate kinase	-
AM340_RS08295	1668676..1670034	-	1359	WP_001302220.1	PTS galactitol transporter subunit IIC	-
AM340_RS08300	1670108..1670389	-	282	WP_000084021.1	PTS sugar transporter subunit IIB	-
AM340_RS08305	1670386..1670859	-	474	WP_001161554.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10167.85 Da
• Isoelectric Point: 10.6128

>T111354 WP_001260303.1 NZ_CP032204:1665343-1665618 [Escherichia coli]
MRTQVQALRKKQKNTLDQIVKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKSAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T111354 NZ_CP032204:1665343-1665618 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAAGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13402.27 Da
• Isoelectric Point: 5.1987

>AT111354 WP_000593555.1 NZ_CP032204:1665615-1665974 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT111354 NZ_CP032204:1665615-1665974 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LKZ6