Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5074533..5075053 Replicon chromosome
Accession NZ_CP032201
Organism Escherichia coli strain AR_0086

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag AM465_RS26295 Protein ID WP_001160964.1
Coordinates 5074533..5074817 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag AM465_RS26300 Protein ID WP_000125566.1
Coordinates 5074820..5075053 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM465_RS26270 5070178..5071464 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
AM465_RS26275 5071464..5072453 + 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
AM465_RS26280 5072450..5073271 + 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
AM465_RS26285 5073274..5073651 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
AM465_RS26290 5073658..5074506 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
AM465_RS26295 5074533..5074817 - 285 WP_001160964.1 CcdB family protein Toxin
AM465_RS26300 5074820..5075053 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
AM465_RS26305 5075139..5075618 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
AM465_RS26310 5075810..5077672 - 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
AM465_RS26315 5077665..5078195 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
AM465_RS26320 5078303..5079634 - 1332 WP_001183209.1 MFS transporter -
AM465_RS26325 5079693..5079980 - 288 WP_000203744.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T111339 WP_001160964.1 NZ_CP032201:c5074817-5074533 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T111339 NZ_CP032201:c5074817-5074533 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT111339 WP_000125566.1 NZ_CP032201:c5075053-5074820 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT111339 NZ_CP032201:c5075053-5074820 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References