Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2852996..2853827 Replicon chromosome
Accession NZ_CP032201
Organism Escherichia coli strain AR_0086

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag AM465_RS14380 Protein ID WP_000854815.1
Coordinates 2852996..2853370 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag AM465_RS14385 Protein ID WP_001280918.1
Coordinates 2853459..2853827 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM465_RS14340 2848392..2849558 + 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
AM465_RS14345 2849677..2850150 + 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
AM465_RS14350 2850348..2851406 + 1059 WP_001200889.1 FUSC family protein -
AM465_RS14355 2851578..2851907 + 330 WP_000450409.1 DUF496 family protein -
AM465_RS14360 2852008..2852190 - 183 WP_001296208.1 hypothetical protein -
AM465_RS14365 2852244..2852390 + 147 Protein_2644 transposase domain-containing protein -
AM465_RS14370 2852679..2852792 - 114 WP_001161660.1 DUF957 domain-containing protein -
AM465_RS14375 2852805..2852999 - 195 WP_000988600.1 hypothetical protein -
AM465_RS14380 2852996..2853370 - 375 WP_000854815.1 type IV toxin-antitoxin system toxin CbtA Toxin
AM465_RS14385 2853459..2853827 - 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
AM465_RS14390 2853843..2854487 - 645 WP_000086752.1 hypothetical protein -
AM465_RS14395 2854506..2854727 - 222 WP_000692345.1 DUF987 domain-containing protein -
AM465_RS14400 2854790..2855266 - 477 WP_001186200.1 RadC family protein -
AM465_RS14405 2855282..2855755 - 474 WP_001542276.1 antirestriction protein -
AM465_RS14410 2855849..2856094 - 246 WP_001164966.1 hypothetical protein -
AM465_RS14415 2856094..2856912 - 819 WP_001542275.1 DUF945 domain-containing protein -
AM465_RS14425 2857133..2857543 - 411 WP_000846703.1 hypothetical protein -
AM465_RS14430 2857559..2857909 - 351 Protein_2656 hypothetical protein -
AM465_RS14440 2857992..2858738 - 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T111326 WP_000854815.1 NZ_CP032201:c2853370-2852996 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T111326 NZ_CP032201:c2853370-2852996 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT111326 WP_001280918.1 NZ_CP032201:c2853827-2853459 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT111326 NZ_CP032201:c2853827-2853459 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References