Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 159534..160270 Replicon plasmid unnamed2
Accession NZ_CP032169
Organism Klebsiella pneumoniae strain AR_0076

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag AM455_RS27990 Protein ID WP_004098919.1
Coordinates 159788..160270 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A455TK27
Locus tag AM455_RS27985 Protein ID WP_020804426.1
Coordinates 159534..159800 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM455_RS27935 155716..156150 + 435 WP_048293723.1 hypothetical protein -
AM455_RS27945 156529..156627 - 99 WP_004118688.1 type II toxin-antitoxin system RelE/ParE family toxin -
AM455_RS27950 156614..156793 - 180 WP_004118349.1 type II toxin-antitoxin system ParD family antitoxin -
AM455_RS27955 157107..157370 + 264 WP_004118691.1 hypothetical protein -
AM455_RS27960 157367..157933 + 567 WP_042922197.1 hypothetical protein -
AM455_RS27965 157964..158458 + 495 WP_016528789.1 hypothetical protein -
AM455_RS27970 158519..158722 + 204 WP_004150739.1 hemolysin expression modulator Hha -
AM455_RS27975 158771..159028 + 258 WP_004098928.1 hypothetical protein -
AM455_RS27980 159104..159358 + 255 WP_032425571.1 hypothetical protein -
AM455_RS27985 159534..159800 + 267 WP_020804426.1 DUF1778 domain-containing protein Antitoxin
AM455_RS27990 159788..160270 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
AM455_RS27995 160471..161874 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
AM455_RS28000 161903..162535 - 633 WP_001567369.1 hypothetical protein -
AM455_RS28005 162761..164107 + 1347 WP_077259381.1 ISNCY family transposase -
AM455_RS28010 164156..164551 + 396 WP_021312679.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..171589 171589


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T111231 WP_004098919.1 NZ_CP032169:159788-160270 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T111231 NZ_CP032169:159788-160270 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGTGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10124.47 Da        Isoelectric Point: 4.3108

>AT111231 WP_020804426.1 NZ_CP032169:159534-159800 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINELL
ARKPQWDV

Download         Length: 267 bp

>AT111231 NZ_CP032169:159534-159800 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCCCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATGAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A455TK27

References