Detailed information of TA system TA111079

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 3113652..3114443
• Replicon: chromosome
• Accession: NZ_CP032085
• Organism: Escherichia coli strain ATCC 25922

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: D1792_RS15040
• Protein ID: WP_000691791.1
• Coordinates: 3114018..3114443 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3L1KVZ6
• Locus tag: D1792_RS15035
• Protein ID: WP_000939437.1
• Coordinates: 3113652..3113987 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1792_RS15010	3109603..3109821	-	219	WP_000070772.1	AlpA family phage regulatory protein	-
D1792_RS15015	3109939..3110553	-	615	WP_000772910.1	inovirus Gp2 family protein	-
D1792_RS15020	3111146..3112099	+	954	WP_000290406.1	hypothetical protein	-
D1792_RS15025	3112335..3113149	+	815	Protein_2950	DUF945 domain-containing protein	-
D1792_RS15030	3113179..3113652	+	474	WP_001292742.1	DNA repair protein RadC	-
D1792_RS15035	3113652..3113987	+	336	WP_000939437.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
D1792_RS15040	3114018..3114443	+	426	WP_000691791.1	TA system toxin CbtA family protein	Toxin
D1792_RS15045	3114464..3115655	+	1192	Protein_2954	AAA family ATPase	-
D1792_RS15050	3115640..3115861	+	222	WP_125112077.1	hypothetical protein	-
D1792_RS15055	3115858..3116589	+	732	WP_001216639.1	TIGR02646 family protein	-
D1792_RS15060	3117212..3117598	+	387	WP_001270938.1	DUF2441 domain-containing protein	-
D1792_RS15065	3118280..3118777	+	498	WP_000509808.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3092990..3118777	25787

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 16490.11 Da
• Isoelectric Point: 10.2376

>T111079 WP_000691791.1 NZ_CP032085:3114018-3114443 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVNAVNFLVEKYELVRIDRKGF
SWQEQTPYLRAVDILRARQATGLRIQCKNTNIISRMADSRHDKRLYQRTFKACKRCYYTKT

Nucleotide

• Length: 426 bp

>T111079 NZ_CP032085:3114018-3114443 [Escherichia coli]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCTCCTGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCAATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGGTCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTGCGGGCGCGGCAGGCGACCGGCCTGCGGATTCAGTG
CAAAAACACGAATATAATCAGCCGGATGGCTGATTCAAGACATGATAAAAGACTTTACCAGCGCACGTTTAAAGCGTGCA
AGAGATGTTATTATACTAAAACATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12556.56 Da
• Isoelectric Point: 7.6977

>AT111079 WP_000939437.1 NZ_CP032085:3113652-3113987 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPCQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT111079 NZ_CP032085:3113652-3113987 [Escherichia coli]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGTTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCCCCTGCCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3L1KVZ6