Detailed information of TA system TA110928

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2686656..2687233
• Replicon: chromosome
• Accession: NZ_CP032011
• Organism: Burkholderia cepacia strain 39628

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C7S14_RS34705
• Protein ID: WP_124469963.1
• Coordinates: 2686656..2687021 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U2HEK9
• Locus tag: C7S14_RS34710
• Protein ID: WP_021160898.1
• Coordinates: 2687015..2687233 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7S14_RS34685	2682303..2682857	+	555	WP_192389062.1	carboxymuconolactone decarboxylase family protein	-
C7S14_RS34690	2683018..2683782	+	765	WP_192389064.1	extensin	-
C7S14_RS34695	2684026..2685624	-	1599	WP_192389066.1	alkyl hydroperoxide reductase subunit F	-
C7S14_RS34700	2685753..2686316	-	564	WP_006488685.1	peroxiredoxin	-
C7S14_RS34705	2686656..2687021	-	366	WP_124469963.1	type II toxin-antitoxin system VapC family toxin	Toxin
C7S14_RS34710	2687015..2687233	-	219	WP_021160898.1	hypothetical protein	Antitoxin
C7S14_RS34715	2687324..2688220	-	897	WP_059237086.1	LysR family transcriptional regulator	-
C7S14_RS34720	2688398..2689564	+	1167	WP_192389068.1	branched-chain amino acid ABC transporter substrate-binding protein	-
C7S14_RS34725	2689624..2690148	+	525	WP_021160901.1	L-2-amino-thiazoline-4-carboxylic acid hydrolase	-
C7S14_RS34730	2690141..2691406	+	1266	WP_192389070.1	Zn-dependent hydrolase	-
C7S14_RS34735	2691494..2691976	-	483	WP_192389072.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13462.53 Da
• Isoelectric Point: 8.4389

>T110928 WP_124469963.1 NZ_CP032011:c2687021-2686656 [Burkholderia cepacia]
MVKALFDTNILIDYLGGVESARAELGRYDYRAISTISWMEVLVGTSAQDEAPIRAWLSSFDVIALDSPIANRAVTIRKER
RIRLPDAIVWASAQVNGLLLVSRNTKDFPATEPGVRVPYKL

Nucleotide

• Length: 366 bp

>T110928 NZ_CP032011:c2687021-2686656 [Burkholderia cepacia]
ATGGTAAAAGCTCTCTTCGATACCAACATCCTGATCGACTATCTGGGCGGCGTGGAATCCGCTCGTGCGGAACTGGGCCG
CTACGACTATCGGGCGATCAGCACGATCTCGTGGATGGAGGTCCTGGTCGGCACGTCCGCACAGGACGAGGCACCCATCC
GCGCGTGGCTGTCGTCGTTCGATGTCATCGCACTGGACAGCCCGATTGCGAATCGTGCCGTGACAATCCGCAAGGAACGC
CGCATACGCCTTCCCGATGCAATCGTCTGGGCATCGGCACAAGTGAACGGTCTACTGCTCGTGTCGCGCAACACGAAGGA
CTTCCCTGCCACCGAACCCGGCGTTCGCGTGCCCTACAAGCTCTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.51 Da
• Isoelectric Point: 5.0604

>AT110928 WP_021160898.1 NZ_CP032011:c2687233-2687015 [Burkholderia cepacia]
MSRILVDLSDGQLDELAVIVETQHRPRAAIIRDAIDAYIALHKHRLADDVFGLWKDRKVDGLTYQEELRSEW

Nucleotide

• Length: 219 bp

>AT110928 NZ_CP032011:c2687233-2687015 [Burkholderia cepacia]
ATGAGCCGAATTCTGGTCGATCTGTCGGACGGCCAACTCGACGAGTTGGCCGTCATCGTCGAGACTCAACATCGTCCCCG
CGCCGCAATCATCCGCGACGCGATCGATGCCTATATTGCCTTGCACAAGCACCGGCTTGCCGACGACGTCTTCGGTCTCT
GGAAAGATCGCAAGGTCGACGGGCTCACCTATCAGGAAGAACTGCGCTCGGAATGGTAA

Structures

Antitoxin

• AlphaFold DB: U2HEK9