Detailed information of TA system TA110836

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 178288..179015
• Replicon: plasmid unnamed1
• Accession: NZ_CP031935
• Organism: Klebsiella pneumoniae strain GZ-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: D1368_RS27995
• Protein ID: WP_011251285.1
• Coordinates: 178704..179015 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D1368_RS27990
• Protein ID: WP_011251286.1
• Coordinates: 178288..178707 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1368_RS27960	173456..173926	-	471	WP_048333570.1	hypothetical protein	-
D1368_RS27965	174119..174874	-	756	Protein_191	hypothetical protein	-
D1368_RS27970	175470..175913	+	444	WP_048333569.1	hypothetical protein	-
D1368_RS27975	175934..176722	+	789	WP_040217257.1	hypothetical protein	-
D1368_RS27980	176736..177071	+	336	Protein_194	conjugal transfer protein TraI	-
D1368_RS27985	177173..178141	-	969	WP_074428168.1	IS5 family transposase	-
D1368_RS27990	178288..178707	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
D1368_RS27995	178704..179015	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
D1368_RS28000	179220..179678	-	459	Protein_198	DDE-type integrase/transposase/recombinase	-
D1368_RS28005	179792..180489	+	698	Protein_199	IS1 family transposase	-
D1368_RS28010	181875..182824	-	950	Protein_200	DUF1173 family protein	-
D1368_RS28015	183002..183496	+	495	WP_004212794.1	thermonuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..198126	198126
	-	inside	IScluster/Tn	-	-	160659..185682	25023

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T110836 WP_011251285.1 NZ_CP031935:c179015-178704 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T110836 NZ_CP031935:c179015-178704 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT110836 WP_011251286.1 NZ_CP031935:c178707-178288 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT110836 NZ_CP031935:c178707-178288 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1