Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3034842..3035467 Replicon chromosome
Accession NZ_CP031934
Organism Klebsiella pneumoniae strain GZ-1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag D1368_RS15025 Protein ID WP_002882817.1
Coordinates 3035084..3035467 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag D1368_RS15020 Protein ID WP_004150355.1
Coordinates 3034842..3035084 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D1368_RS14995 3030833..3031432 + 600 WP_004151865.1 glucose-1-phosphatase -
D1368_RS15000 3031426..3032286 + 861 WP_002882807.1 virulence factor BrkB family protein -
D1368_RS15005 3032283..3032720 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
D1368_RS15010 3032765..3033706 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
D1368_RS15015 3033720..3034637 - 918 WP_002882812.1 alpha/beta hydrolase -
D1368_RS15020 3034842..3035084 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
D1368_RS15025 3035084..3035467 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
D1368_RS15030 3035641..3036570 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
D1368_RS15035 3036567..3037202 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
D1368_RS15040 3037199..3038101 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T110828 WP_002882817.1 NZ_CP031934:3035084-3035467 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T110828 NZ_CP031934:3035084-3035467 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT110828 WP_004150355.1 NZ_CP031934:3034842-3035084 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT110828 NZ_CP031934:3034842-3035084 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References