Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 48868..49393 Replicon plasmid unnamed13
Accession NZ_CP031914
Organism Escherichia coli O157:H7 strain FWSEC0004

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag CAM50_RS27645 Protein ID WP_001159868.1
Coordinates 49088..49393 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag CAM50_RS27640 Protein ID WP_000813634.1
Coordinates 48868..49086 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CAM50_RS27605 44132..44362 + 231 WP_010891288.1 hypothetical protein -
CAM50_RS27610 44483..45223 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
CAM50_RS27615 45508..46485 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
CAM50_RS27625 46893..47093 - 201 WP_000708307.1 hypothetical protein -
CAM50_RS27630 47090..47710 - 621 WP_001248529.1 hypothetical protein -
CAM50_RS27635 47939..48409 - 471 Protein_45 DNA-binding protein -
CAM50_RS27640 48868..49086 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CAM50_RS27645 49088..49393 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
CAM50_RS27650 49394..50200 + 807 WP_000016989.1 site-specific integrase -
CAM50_RS27665 52098..52436 + 339 WP_071525396.1 RepB family plasmid replication initiator protein -
CAM50_RS27670 53024..54190 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB 1..92754 92754


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T110722 WP_001159868.1 NZ_CP031914:49088-49393 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T110722 NZ_CP031914:49088-49393 [Escherichia coli O157:H7]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT110722 WP_000813634.1 NZ_CP031914:48868-49086 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT110722 NZ_CP031914:48868-49086 [Escherichia coli O157:H7]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References