Detailed information of TA system TA110455

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 2067346..2068061
• Replicon: chromosome
• Accession: NZ_CP031873
• Organism: Actinobacillus pleuropneumoniae serovar 4 str. M62

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D1097_RS09840
• Protein ID: WP_005606490.1
• Coordinates: 2067346..2067711 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BT01
• Locus tag: D1097_RS09845
• Protein ID: WP_005599447.1
• Coordinates: 2067774..2068061 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1097_RS09825 (D1097_09875)	2062931..2064637	-	1707	WP_005606484.1	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	-
D1097_RS09830 (D1097_09880)	2064733..2065014	-	282	WP_005599441.1	DNA-directed RNA polymerase subunit omega	-
D1097_RS09835 (D1097_09885)	2065141..2067222	-	2082	WP_005606488.1	ATP-dependent DNA helicase RecG	-
D1097_RS09840 (D1097_09890)	2067346..2067711	-	366	WP_005606490.1	type II toxin-antitoxin system VapC family toxin	Toxin
D1097_RS09845 (D1097_09895)	2067774..2068061	-	288	WP_005599447.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
D1097_RS09850 (D1097_09900)	2068267..2069982	+	1716	WP_005606494.1	proline--tRNA ligase	-
D1097_RS09855 (D1097_09905)	2070048..2070236	-	189	WP_005602684.1	hypothetical protein	-
D1097_RS09860 (D1097_09910)	2070239..2070820	-	582	WP_005606496.1	sigma-70 family RNA polymerase sigma factor	-
D1097_RS09865 (D1097_09915)	2070909..2071865	+	957	WP_005619040.1	protein-methionine-sulfoxide reductase catalytic subunit MsrP	-
D1097_RS09870 (D1097_09920)	2071865..2072458	+	594	WP_005606498.1	protein-methionine-sulfoxide reductase heme-binding subunit MsrQ	-
D1097_RS09875 (D1097_09925)	2072650..2072868	-	219	Protein_1899	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13921.01 Da
• Isoelectric Point: 8.2783

>T110455 WP_005606490.1 NZ_CP031873:c2067711-2067346 [Actinobacillus pleuropneumoniae serovar 4 str. M62]
MDTNVRQWLATIKPSQTNISCITLSEIKTGILLKARKDPIQAERLNHWFTHNVLSVYQAKSFSINNEIALLASEYHIPNK
MDLNDAYIAATAKYHNLVLVTRNLKDFNCCDIRLFNPFEPN

Nucleotide

• Length: 366 bp

>T110455 NZ_CP031873:c2067711-2067346 [Actinobacillus pleuropneumoniae serovar 4 str. M62]
ATGGATACCAATGTACGACAATGGTTAGCAACGATTAAACCGAGTCAGACGAATATTAGCTGTATCACGCTTTCCGAAAT
AAAAACGGGGATTTTACTCAAAGCTCGTAAAGATCCTATTCAAGCCGAGCGGTTAAATCATTGGTTTACCCATAATGTAT
TAAGCGTTTATCAAGCTAAATCCTTTTCTATAAATAATGAAATTGCGTTACTGGCTTCTGAATACCACATTCCAAATAAA
ATGGATTTAAACGATGCTTATATTGCCGCAACCGCTAAATATCATAATTTAGTGCTTGTTACTCGTAATTTGAAAGACTT
CAACTGTTGCGATATACGTTTATTTAATCCTTTCGAACCGAATTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11040.29 Da
• Isoelectric Point: 4.5578

>AT110455 WP_005599447.1 NZ_CP031873:c2068061-2067774 [Actinobacillus pleuropneumoniae serovar 4 str. M62]
MPITITSREFNQHASKVLKMAEKEPVFITKWGKIVSVVSNYVDYKQHTTVEPSFEELFASSTDESVSDEFWQDFEQTLTK
IRKSNKSEQIDFGDE

Nucleotide

• Length: 288 bp

>AT110455 NZ_CP031873:c2068061-2067774 [Actinobacillus pleuropneumoniae serovar 4 str. M62]
ATGCCAATTACTATTACCTCTCGAGAATTTAATCAACACGCCAGTAAAGTGCTGAAAATGGCAGAAAAAGAACCGGTTTT
TATCACTAAATGGGGAAAAATTGTCAGCGTTGTCTCCAATTATGTTGATTACAAACAGCACACAACGGTTGAGCCTAGCT
TTGAAGAACTATTTGCCTCATCTACGGATGAATCGGTTAGTGATGAGTTTTGGCAAGATTTTGAACAGACATTAACAAAA
ATTCGCAAAAGTAATAAATCCGAACAAATAGATTTTGGAGATGAATAA

Structures

Antitoxin

• AlphaFold DB: A3N3C5