Detailed information of TA system TA110427

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 2100385..2101100
• Replicon: chromosome
• Accession: NZ_CP031863
• Organism: Actinobacillus pleuropneumoniae serovar 11 str. 56153

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D1104_RS09990
• Protein ID: WP_005599445.1
• Coordinates: 2100385..2100750 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BT01
• Locus tag: D1104_RS09995
• Protein ID: WP_005599447.1
• Coordinates: 2100813..2101100 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1104_RS09975 (D1104_10045)	2095970..2097676	-	1707	WP_005599433.1	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	-
D1104_RS09980 (D1104_10050)	2097772..2098053	-	282	WP_005599441.1	DNA-directed RNA polymerase subunit omega	-
D1104_RS09985 (D1104_10055)	2098180..2100261	-	2082	WP_005599444.1	ATP-dependent DNA helicase RecG	-
D1104_RS09990 (D1104_10060)	2100385..2100750	-	366	WP_005599445.1	type II toxin-antitoxin system VapC family toxin	Toxin
D1104_RS09995 (D1104_10065)	2100813..2101100	-	288	WP_005599447.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
D1104_RS10000 (D1104_10070)	2101306..2103021	+	1716	WP_005599448.1	proline--tRNA ligase	-
D1104_RS10005 (D1104_10075)	2103087..2103275	-	189	WP_005602684.1	hypothetical protein	-
D1104_RS10010 (D1104_10080)	2103278..2103859	-	582	WP_005599450.1	sigma-70 family RNA polymerase sigma factor	-
D1104_RS10015 (D1104_10085)	2103948..2104904	+	957	WP_005619040.1	protein-methionine-sulfoxide reductase catalytic subunit MsrP	-
D1104_RS10020 (D1104_10090)	2104904..2105497	+	594	WP_005599453.1	protein-methionine-sulfoxide reductase heme-binding subunit MsrQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13974.06 Da
• Isoelectric Point: 9.0549

>T110427 WP_005599445.1 NZ_CP031863:c2100750-2100385 [Actinobacillus pleuropneumoniae serovar 11 str. 56153]
MDTNVRQWLATIKPSQTNISCITLSEIKTGILLKARKDPIQAERLNHWFTHNVLSVYQAKSFSINNEIALLASEYHIPNK
MDLNDAYIAATAKYHNLVLVTRNLKDFNRCDIRLFNPFEPN

Nucleotide

• Length: 366 bp

>T110427 NZ_CP031863:c2100750-2100385 [Actinobacillus pleuropneumoniae serovar 11 str. 56153]
ATGGATACCAATGTACGACAATGGTTAGCAACGATTAAACCGAGTCAGACGAATATTAGCTGTATCACGCTTTCCGAAAT
AAAAACGGGGATTTTACTCAAAGCTCGTAAAGATCCTATTCAAGCCGAGCGGTTAAATCATTGGTTTACCCATAATGTAT
TAAGCGTTTATCAAGCTAAATCCTTTTCTATAAATAATGAAATTGCGTTACTGGCTTCTGAATACCACATTCCAAATAAA
ATGGATTTAAACGATGCTTATATTGCCGCAACCGCTAAATATCATAATTTAGTGCTTGTTACTCGTAATTTGAAAGACTT
CAACCGTTGCGATATACGTTTATTTAATCCTTTCGAACCGAATTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11040.29 Da
• Isoelectric Point: 4.5578

>AT110427 WP_005599447.1 NZ_CP031863:c2101100-2100813 [Actinobacillus pleuropneumoniae serovar 11 str. 56153]
MPITITSREFNQHASKVLKMAEKEPVFITKWGKIVSVVSNYVDYKQHTTVEPSFEELFASSTDESVSDEFWQDFEQTLTK
IRKSNKSEQIDFGDE

Nucleotide

• Length: 288 bp

>AT110427 NZ_CP031863:c2101100-2100813 [Actinobacillus pleuropneumoniae serovar 11 str. 56153]
ATGCCAATTACTATTACCTCTCGAGAATTTAATCAACACGCCAGTAAAGTGCTGAAAATGGCAGAAAAAGAACCGGTTTT
TATCACTAAATGGGGAAAAATTGTCAGCGTTGTCTCCAATTATGTTGATTACAAACAGCACACAACGGTTGAGCCTAGCT
TTGAAGAACTATTTGCCTCATCTACGGATGAATCGGTTAGCGATGAGTTTTGGCAAGATTTTGAACAGACATTAACAAAA
ATTCGCAAAAGTAATAAATCCGAACAAATAGATTTTGGAGATGAATAA

Structures

Antitoxin

• AlphaFold DB: A3N3C5