Detailed information of TA system TA109961

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 42414..43051
• Replicon: plasmid p2_020038
• Accession: NZ_CP031723
• Organism: Enterobacter hormaechei strain WCHEH020038

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A9E6WXZ4
• Locus tag: D0Z05_RS00940
• Protein ID: WP_032662169.1
• Coordinates: 42414..42824 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A9E6WTF5
• Locus tag: D0Z05_RS00945
• Protein ID: WP_032662167.1
• Coordinates: 42821..43051 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0Z05_RS00920	37780..38130	+	351	WP_032662182.1	DUF305 domain-containing protein	-
D0Z05_RS00925	38316..39224	-	909	WP_032662179.1	HNH endonuclease	-
D0Z05_RS00930	39771..40793	-	1023	WP_032662174.1	helicase UvrD	-
D0Z05_RS00935	40778..42340	-	1563	WP_032662171.1	AAA family ATPase	-
D0Z05_RS00940	42414..42824	-	411	WP_032662169.1	PIN domain-containing protein	Toxin
D0Z05_RS00945	42821..43051	-	231	WP_032662167.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
D0Z05_RS00955	43593..43883	+	291	WP_013087126.1	hypothetical protein	-
D0Z05_RS00960	44014..44544	+	531	WP_013087127.1	hypothetical protein	-
D0Z05_RS00965	44563..45339	+	777	WP_015572054.1	site-specific integrase	-
D0Z05_RS00970	45541..45798	+	258	Protein_45	site-specific integrase	-
D0Z05_RS00975	46042..47052	-	1011	WP_015572055.1	RepB family plasmid replication initiator protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(D) / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B	-	1..88009	88009

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-127)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14729.03 Da
• Isoelectric Point: 5.7351

>T109961 WP_032662169.1 NZ_CP031723:c42824-42414 [Enterobacter hormaechei]
VNKMYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITWAEVSLASQEAGPAAQKLADAFAARLDAILPWDRTAV
DATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVR

Nucleotide

• Length: 411 bp

>T109961 NZ_CP031723:c42824-42414 [Enterobacter hormaechei]
GTGAACAAAATGTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGGGGTCACCGCATTGTGGTTTCGGCCATCACCTGGGCCGAAGTGTCACTGGCATCACAGGAAG
CAGGACCAGCCGCACAGAAGCTGGCTGACGCCTTCGCTGCCCGCCTTGATGCCATCCTGCCCTGGGACCGTACCGCGGTG
GACGCGACCACGGAAATTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGGCCGAACGACACGGCGATCGCCGGGCA
CGCCATCGCCGCCGGTGCCGTGCTGGTGACCAATAATGTGCGGGAATTTGAGCGGGTGCCGGGTCTGGTGCTGGAAGACT
GGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8645.77 Da
• Isoelectric Point: 4.4692

>AT109961 WP_032662167.1 NZ_CP031723:c43051-42821 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMAERGDVVSDEGRVNL

Nucleotide

• Length: 231 bp

>AT109961 NZ_CP031723:c43051-42821 [Enterobacter hormaechei]
ATGAGAACCGTATCTATTTTCAAAAACGGCAACAACCGCGCCATCCGCCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCCTGGAGCTCG
AAAAAGCTGACCCGGACTTTATGGCTGAGCGCGGGGACGTTGTCAGCGACGAAGGACGGGTTAACCTGTGA