Detailed information of TA system TA109875

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1768646..1769391
• Replicon: chromosome
• Accession: NZ_CP031695
• Organism: Erwinia billingiae strain TH88

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: D0N50_RS07990
• Protein ID: WP_151037049.1
• Coordinates: 1768900..1769391 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: D0N50_RS07985
• Protein ID: WP_151037048.1
• Coordinates: 1768646..1768912 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0N50_RS07960	1764073..1765095	+	1023	WP_151037044.1	isopenicillin N synthase family oxygenase	-
D0N50_RS07965	1765112..1765915	+	804	WP_053142248.1	MetQ/NlpA family ABC transporter substrate-binding protein	-
D0N50_RS07970	1765908..1766696	+	789	WP_151037045.1	ATP-binding cassette domain-containing protein	-
D0N50_RS07975	1766684..1767352	+	669	WP_151037046.1	ABC transporter permease	-
D0N50_RS07980	1767371..1768441	+	1071	WP_151037047.1	iron-containing alcohol dehydrogenase family protein	-
D0N50_RS07985	1768646..1768912	+	267	WP_151037048.1	DUF1778 domain-containing protein	Antitoxin
D0N50_RS07990	1768900..1769391	+	492	WP_151037049.1	GNAT family N-acetyltransferase	Toxin
D0N50_RS07995	1769482..1769571	+	90	Protein_1566	VapC toxin family PIN domain ribonuclease	-
D0N50_RS08000	1769665..1770825	+	1161	WP_151036249.1	IS30 family transposase	-
D0N50_RS08005	1770862..1771443	-	582	WP_151038685.1	HNH endonuclease	-
D0N50_RS08010	1772323..1772961	+	639	WP_134824464.1	NAD(P)H-dependent oxidoreductase	-
D0N50_RS08015	1773069..1773185	+	117	Protein_1570	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1769665..1770825	1160

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-147)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17530.26 Da
• Isoelectric Point: 7.8880

>T109875 WP_151037049.1 NZ_CP031695:1768900-1769391 [Erwinia billingiae]
VGKVTAPAPLNTTHILSEFHSGEAALDEWIRLRGLKNQSTGAARTFVVCLEDSSHVVGFYSLATGSVTHAIAPGGLRRNM
PDPLPIIILARLAVDSSCQGQGLGADLLHDAVLRICRVAENIGVRAIMVHALSDVAKQFYLHHGFQPSMTQDKTLFLRLP
TAI

Nucleotide

• Length: 492 bp

>T109875 NZ_CP031695:1768900-1769391 [Erwinia billingiae]
GTGGGAAAAGTAACCGCTCCAGCACCTCTAAATACGACTCACATTCTTTCAGAGTTTCACTCCGGGGAAGCTGCACTGGA
TGAATGGATCAGGCTCAGAGGGTTAAAAAACCAGTCGACCGGCGCCGCCAGAACGTTTGTGGTGTGCCTAGAAGATTCTT
CACATGTGGTTGGTTTTTACTCTCTGGCCACCGGCAGTGTCACTCATGCCATTGCACCCGGTGGGCTCAGACGAAACATG
CCTGACCCTCTTCCCATCATAATTTTGGCCCGGCTGGCAGTTGATTCGAGTTGCCAGGGCCAGGGTCTTGGTGCGGATTT
ACTGCACGATGCAGTACTCCGTATTTGCCGGGTTGCGGAGAATATTGGCGTCCGGGCCATTATGGTGCATGCCTTGTCTG
ATGTGGCAAAACAGTTTTACCTGCATCATGGATTCCAGCCCTCAATGACACAGGATAAAACACTTTTTCTCCGGTTACCT
ACTGCCATTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9999.52 Da
• Isoelectric Point: 5.0757

>AT109875 WP_151037048.1 NZ_CP031695:1768646-1768912 [Erwinia billingiae]
MKSDVQLNIRAKEAQRALIDTAAAILHKSRTDFILDIACQAAENVILDRRMFNLDDKQYAEFIDMLDAPVTANPALDTLL
ARKPQWEK

Nucleotide

• Length: 267 bp

>AT109875 NZ_CP031695:1768646-1768912 [Erwinia billingiae]
ATGAAATCTGATGTTCAGCTTAACATTCGGGCTAAAGAAGCCCAGCGCGCGTTGATCGATACCGCTGCCGCTATTCTTCA
TAAATCACGCACCGACTTTATACTAGACATCGCTTGCCAGGCTGCTGAAAACGTTATCCTTGATCGCCGGATGTTTAATC
TCGATGATAAACAGTACGCCGAGTTTATCGATATGCTGGATGCACCCGTTACAGCGAATCCGGCACTGGATACGCTGCTG
GCGAGAAAACCGCAGTGGGAAAAGTAA