Detailed information of TA system TA109653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5520478..5521073
• Replicon: chromosome
• Accession: NZ_CP031659
• Organism: Pseudomonas aeruginosa strain PABL012

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: DZ894_RS26495
• Protein ID: WP_003113526.1
• Coordinates: 5520795..5521073 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DZ894_RS26490
• Protein ID: WP_003099268.1
• Coordinates: 5520478..5520783 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DZ894_RS26455	5515618..5516466	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
DZ894_RS26465	5516633..5517574	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
DZ894_RS26470	5517691..5518305	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
DZ894_RS26475	5518347..5518931	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
DZ894_RS26480	5518972..5520072	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
DZ894_RS26490	5520478..5520783	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
DZ894_RS26495	5520795..5521073	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DZ894_RS26505	5521402..5523630	+	2229	WP_021264080.1	TonB-dependent receptor	-
DZ894_RS26510	5523700..5524347	-	648	WP_003095021.1	carbonate dehydratase	-
DZ894_RS26515	5524409..5525647	-	1239	WP_019681675.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T109653 WP_003113526.1 NZ_CP031659:c5521073-5520795 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T109653 NZ_CP031659:c5521073-5520795 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGTTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT109653 WP_003099268.1 NZ_CP031659:c5520783-5520478 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT109653 NZ_CP031659:c5520783-5520478 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3