Detailed information of TA system TA109645

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 54077..54341
• Replicon: plasmid pCARB35_03
• Accession: NZ_CP031656
• Organism: Escherichia coli strain UK_Dog_Liverpool

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: DL545_RS26720
• Protein ID: WP_001387489.1
• Coordinates: 54077..54229 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	54281..54341 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DL545_RS27100	49642..49758	+	117	Protein_58	IncI1-type conjugal transfer lipoprotein TraH	-
DL545_RS26695	49757..51511	+	1755	Protein_59	DotA/TraY family protein	-
DL545_RS26700	51582..52244	+	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
DL545_RS26705	52316..52525	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
DL545_RS27105	52917..53093	+	177	WP_001054897.1	hypothetical protein	-
DL545_RS26710	53158..53454	-	297	WP_011264046.1	hypothetical protein	-
DL545_RS26715	53754..54005	+	252	WP_001291964.1	hypothetical protein	-
DL545_RS26720	54077..54229	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
-	54281..54341	+	61	NuclAT_0	-	Antitoxin
-	54281..54341	+	61	NuclAT_0	-	Antitoxin
-	54281..54341	+	61	NuclAT_0	-	Antitoxin
-	54281..54341	+	61	NuclAT_0	-	Antitoxin
DL545_RS26725	54544..54891	+	348	Protein_66	protein finQ	-
DL545_RS26730	55077..56234	+	1158	Protein_67	IS1380-like element ISEc9 family transposase	-
DL545_RS26740	56290..56987	+	698	Protein_68	IS1 family transposase	-
DL545_RS26745	57001..57306	-	306	Protein_69	transcription termination factor NusG	-
DL545_RS26755	57748..58035	-	288	WP_000074855.1	conjugal transfer protein TraA	-
DL545_RS26760	57953..58216	-	264	WP_032180564.1	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-42	-	1..59142	59142

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T109645 WP_001387489.1 NZ_CP031656:c54229-54077 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T109645 NZ_CP031656:c54229-54077 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT109645 NZ_CP031656:54281-54341 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin