Detailed information of TA system TA109620

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 181924..182178
• Replicon: chromosome
• Accession: NZ_CP031653
• Organism: Escherichia coli strain UK_Dog_Liverpool

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: DL545_RS00960
• Protein ID: WP_015387399.1
• Coordinates: 181972..182178 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	181924..181985 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DL545_RS00910	177576..177788	+	213	WP_005012601.1	hypothetical protein	-
DL545_RS27120	178089..178178	-	90	Protein_170	IS1 family transposase	-
DL545_RS00925	178233..178910	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
DL545_RS00930	178910..179257	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
DL545_RS00935	179277..180848	+	1572	WP_023149734.1	IS66 family transposase	-
DL545_RS00940	180886..181502	-	617	Protein_174	IS1 family transposase	-
DL545_RS00950	181603..181785	+	183	WP_000968309.1	hypothetical protein	-
-	181924..181985	-	62	-	-	Antitoxin
DL545_RS00960	181972..182178	+	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
DL545_RS00965	182462..182719	+	258	WP_000083833.1	replication regulatory protein RepA	-
DL545_RS00975	182955..183029	+	75	WP_032336874.1	RepA leader peptide Tap	-
DL545_RS00980	183022..183468	+	447	Protein_179	incFII family plasmid replication initiator RepA	-
DL545_RS00985	183414..183896	-	483	Protein_180	VENN motif pre-toxin domain-containing protein	-
DL545_RS00990	184789..186009	+	1221	WP_000410951.1	arginine deiminase	-
DL545_RS00995	186020..186931	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T109620 WP_015387399.1 NZ_CP031653:181972-182178 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T109620 NZ_CP031653:181972-182178 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT109620 NZ_CP031653:c181985-181924 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin