Detailed information of TA system TA109616

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sib/-
• Location: 164911..165144
• Replicon: chromosome
• Accession: NZ_CP031653
• Organism: Escherichia coli strain UK_Dog_Liverpool

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: DL545_RS00830
• Protein ID: WP_001312861.1
• Coordinates: 164986..165144 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sib
Locus tag	-
Coordinates	164911..164942 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DL545_RS00815	160226..161137	+	912	WP_001168544.1	glycine--tRNA ligase subunit alpha	-
DL545_RS00820	161147..163216	+	2070	WP_001291774.1	glycine--tRNA ligase subunit beta	-
DL545_RS00825	163517..164886	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
-	164911..164942	+	32	NuclAT_1	-	Antitoxin
-	164911..164942	+	32	NuclAT_1	-	Antitoxin
-	164911..164942	+	32	NuclAT_1	-	Antitoxin
-	164911..164942	+	32	NuclAT_1	-	Antitoxin
DL545_RS00830	164986..165144	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
DL545_RS00845	166065..166352	+	288	WP_000107535.1	hypothetical protein	-
DL545_RS00850	166470..167291	+	822	WP_001234426.1	DUF945 domain-containing protein	-
DL545_RS00855	167588..168190	-	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
DL545_RS00860	168511..168894	+	384	WP_001151524.1	relaxosome protein TraM	-
DL545_RS00865	169081..169770	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T109616 WP_001312861.1 NZ_CP031653:164986-165144 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T109616 NZ_CP031653:164986-165144 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 32 bp

>AT109616 NZ_CP031653:164911-164942 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin