109494

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 36283..36805 Replicon plasmid pIncHI1B-1502320
Accession NZ_CP031582
Organism Klebsiella pneumoniae strain N4b

Toxin (Protein)


Gene name stbE Uniprot ID A0A4R0GPV2
Locus tag AXA58_RS27940 Protein ID WP_032731992.1
Coordinates 36521..36805 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A2J4ZSR4
Locus tag AXA58_RS27935 Protein ID WP_004187025.1
Coordinates 36283..36531 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AXA58_RS27920 31701..32834 - 1134 WP_022651297.1 glutathione-independent formaldehyde dehydrogenase -
AXA58_RS27925 33293..34891 - 1599 WP_050597293.1 sensor domain-containing diguanylate cyclase -
AXA58_RS27930 35115..36038 + 924 WP_032731993.1 IS5 family transposase -
AXA58_RS27935 36283..36531 + 249 WP_004187025.1 plasmid stabilization protein Antitoxin
AXA58_RS27940 36521..36805 + 285 WP_032731992.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AXA58_RS27945 37040..37698 + 659 Protein_35 IS1 family transposase -
AXA58_RS27950 37747..38730 - 984 Protein_36 IS630 family transposase -
AXA58_RS27955 39085..39462 + 378 Protein_37 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid formA - 1..206229 206229
- inside IScluster/Tn formA - 28349..46803 18454


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 11017.75 Da        Isoelectric Point: 10.5388

>T109494 WP_032731992.1 NZ_CP031582:36521-36805 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGIGK
RENDDIYNATLNRN

Download         Length: 285 bp

>T109494 NZ_CP031582:36521-36805 [Klebsiella pneumoniae]
ATGACCTATAAGCTGGCTTTCAACGAATCCGCGCTGAAAGAATGGAAGAAGCTGGGTCATACACTACAGGTACAGTTCAA
AAAGAAGCTCAAAGAGCGCCTGGAGAACCCCAGAGTTCCGGCATCACAGCTACATGGACGTAAGGACCAGTATAAAATTA
AGCTACGTGGTGCCGGATACCGCCTTGTCTATCGTGTAGAGGACGACATTGTCACAGTCACTGTTATCGGCATAGGCAAA
CGCGAAAACGACGACATTTATAATGCAACCCTTAATCGCAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9011.24 Da        Isoelectric Point: 3.9947

>AT109494 WP_004187025.1 NZ_CP031582:36283-36531 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Download         Length: 249 bp

>AT109494 NZ_CP031582:36283-36531 [Klebsiella pneumoniae]
ATGGCATTTCAGGTTCTGACCACTACAGCAGCTAGCATTACTGAACTTAAACGTGACCCTATGGGTACTTTCCACGCCGG
GGATGGCGCTCCGGTAGCCATTCTTAATCGTAATGAGCCTGCATTCTACTGTGTGCCACCGGCACTCTATGCCCAGTTGA
TGGAGCTTCTGGAAGATGAAGAGCTGGGACGGATGGCTGATGAGCGAAGCGGAGAAAAGGTTATAGAGGTCAATATTGAT
GACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4R0GPV2

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZSR4

References