Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2633068..2633570 Replicon chromosome
Accession NZ_CP031347
Organism Escherichia coli O145 strain RM9873-C1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DXE52_RS13720 Protein ID WP_000767829.1
Coordinates 2633068..2633322 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DXE52_RS13725 Protein ID WP_001259255.1
Coordinates 2633319..2633570 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DXE52_RS13690 2628083..2628310 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
DXE52_RS13695 2628324..2629382 - 1059 WP_000492332.1 transport protein YeeE -
DXE52_RS13700 2629561..2630919 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DXE52_RS13705 2630909..2630971 - 63 WP_010723108.1 membrane protein YoeI -
DXE52_RS13710 2631186..2632115 - 930 WP_000803351.1 LysR family transcriptional regulator -
DXE52_RS13715 2632161..2632985 - 825 WP_000754755.1 SDR family oxidoreductase -
DXE52_RS13720 2633068..2633322 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DXE52_RS13725 2633319..2633570 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DXE52_RS13730 2633854..2633904 + 51 WP_001364200.1 his operon leader peptide -
DXE52_RS13735 2634050..2634949 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
DXE52_RS13740 2634955..2636259 + 1305 WP_001400693.1 histidinol dehydrogenase -
DXE52_RS13745 2636256..2637326 + 1071 WP_001099213.1 histidinol-phosphate transaminase -
DXE52_RS13750 2637326..2638393 + 1068 WP_000080083.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T109038 WP_000767829.1 NZ_CP031347:c2633322-2633068 [Escherichia coli O145]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T109038 NZ_CP031347:c2633322-2633068 [Escherichia coli O145]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT109038 WP_001259255.1 NZ_CP031347:c2633570-2633319 [Escherichia coli O145]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT109038 NZ_CP031347:c2633570-2633319 [Escherichia coli O145]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References