Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1913674..1914312 Replicon chromosome
Accession NZ_CP031347
Organism Escherichia coli O145 strain RM9873-C1

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag DXE52_RS09800 Protein ID WP_000813794.1
Coordinates 1914136..1914312 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag DXE52_RS09795 Protein ID WP_001270286.1
Coordinates 1913674..1914090 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DXE52_RS09775 1908825..1909766 - 942 WP_001251328.1 ABC transporter permease -
DXE52_RS09780 1909767..1910780 - 1014 WP_000220414.1 ABC transporter ATP-binding protein -
DXE52_RS09785 1910798..1911943 - 1146 WP_000335342.1 ABC transporter substrate-binding protein -
DXE52_RS09790 1912189..1913595 - 1407 WP_000760665.1 PLP-dependent aminotransferase family protein -
DXE52_RS09795 1913674..1914090 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
DXE52_RS09800 1914136..1914312 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
DXE52_RS09805 1914534..1914764 + 231 WP_000494244.1 YncJ family protein -
DXE52_RS09810 1914856..1916817 - 1962 WP_000218823.1 23S rRNA 5-hydroxycytidine C2501 synthase -
DXE52_RS09815 1916890..1917426 - 537 WP_000429147.1 DNA-binding transcriptional regulator SutR -
DXE52_RS09820 1917479..1918690 + 1212 WP_071792349.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T109029 WP_000813794.1 NZ_CP031347:c1914312-1914136 [Escherichia coli O145]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T109029 NZ_CP031347:c1914312-1914136 [Escherichia coli O145]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT109029 WP_001270286.1 NZ_CP031347:c1914090-1913674 [Escherichia coli O145]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT109029 NZ_CP031347:c1914090-1913674 [Escherichia coli O145]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCATTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References