Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2727026..2727528 Replicon chromosome
Accession NZ_CP031345
Organism Escherichia coli O145 strain RM12367-C1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DXE53_RS14380 Protein ID WP_000767829.1
Coordinates 2727026..2727280 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DXE53_RS14385 Protein ID WP_001259255.1
Coordinates 2727277..2727528 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DXE53_RS14350 2722041..2722268 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
DXE53_RS14355 2722282..2723340 - 1059 WP_000492332.1 transport protein YeeE -
DXE53_RS14360 2723519..2724877 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DXE53_RS14365 2724867..2724929 - 63 WP_010723108.1 membrane protein YoeI -
DXE53_RS14370 2725144..2726073 - 930 WP_000803351.1 LysR family transcriptional regulator -
DXE53_RS14375 2726119..2726943 - 825 WP_000754755.1 SDR family oxidoreductase -
DXE53_RS14380 2727026..2727280 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DXE53_RS14385 2727277..2727528 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DXE53_RS14390 2727812..2727862 + 51 WP_001364200.1 his operon leader peptide -
DXE53_RS14395 2728008..2728907 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
DXE53_RS14400 2728913..2730217 + 1305 WP_001400693.1 histidinol dehydrogenase -
DXE53_RS14405 2730214..2731284 + 1071 WP_001099213.1 histidinol-phosphate transaminase -
DXE53_RS14410 2731284..2732351 + 1068 WP_000080083.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T109009 WP_000767829.1 NZ_CP031345:c2727280-2727026 [Escherichia coli O145]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T109009 NZ_CP031345:c2727280-2727026 [Escherichia coli O145]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT109009 WP_001259255.1 NZ_CP031345:c2727528-2727277 [Escherichia coli O145]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT109009 NZ_CP031345:c2727528-2727277 [Escherichia coli O145]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References