Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 484812..485430 Replicon chromosome
Accession NZ_CP031345
Organism Escherichia coli O145 strain RM12367-C1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag DXE53_RS02685 Protein ID WP_001291435.1
Coordinates 484812..485030 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag DXE53_RS02690 Protein ID WP_000344800.1
Coordinates 485056..485430 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DXE53_RS02650 480101..480673 + 573 WP_000779831.1 YbaY family lipoprotein -
DXE53_RS02655 480704..481015 - 312 WP_000409911.1 MGMT family protein -
DXE53_RS02665 481394..481747 + 354 WP_000878140.1 DUF1428 domain-containing protein -
DXE53_RS02670 481789..483339 - 1551 WP_024221674.1 cyclic-guanylate-specific phosphodiesterase PdeB -
DXE53_RS02675 483503..483973 - 471 WP_000136192.1 YlaC family protein -
DXE53_RS02680 484089..484640 - 552 WP_000102564.1 maltose O-acetyltransferase -
DXE53_RS02685 484812..485030 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
DXE53_RS02690 485056..485430 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
DXE53_RS02695 485976..489125 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
DXE53_RS02700 489148..490341 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T108993 WP_001291435.1 NZ_CP031345:c485030-484812 [Escherichia coli O145]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T108993 NZ_CP031345:c485030-484812 [Escherichia coli O145]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT108993 WP_000344800.1 NZ_CP031345:c485430-485056 [Escherichia coli O145]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT108993 NZ_CP031345:c485430-485056 [Escherichia coli O145]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References