Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2123836..2124454 Replicon chromosome
Accession NZ_CP031215
Organism Escherichia coli strain Es_ST80_L1_NDM_10_2017

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag DV870_RS11040 Protein ID WP_001291435.1
Coordinates 2124236..2124454 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag DV870_RS11035 Protein ID WP_000344800.1
Coordinates 2123836..2124210 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DV870_RS11025 2118925..2120118 + 1194 WP_001538394.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
DV870_RS11030 2120141..2123290 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
DV870_RS11035 2123836..2124210 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
DV870_RS11040 2124236..2124454 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
DV870_RS11045 2124627..2125178 + 552 WP_000102539.1 maltose O-acetyltransferase -
DV870_RS11050 2125294..2125764 + 471 WP_000136192.1 YlaC family protein -
DV870_RS11055 2125928..2127478 + 1551 WP_001538392.1 cyclic-guanylate-specific phosphodiesterase PdeB -
DV870_RS11060 2127520..2127873 - 354 WP_000878140.1 DUF1428 domain-containing protein -
DV870_RS11070 2128252..2128563 + 312 WP_000409908.1 MGMT family protein -
DV870_RS11075 2128594..2129166 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T108608 WP_001291435.1 NZ_CP031215:2124236-2124454 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T108608 NZ_CP031215:2124236-2124454 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT108608 WP_000344800.1 NZ_CP031215:2123836-2124210 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT108608 NZ_CP031215:2123836-2124210 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References