108165

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 655432..656046 Replicon chromosome
Accession NZ_CP030913
Organism Pseudomonas aeruginosa strain Y89

Toxin (Protein)


Gene name hicA Uniprot ID A6VBH9
Locus tag Y89_RS03110 Protein ID WP_071534354.1
Coordinates 655432..655614 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A140SDX7
Locus tag Y89_RS03115 Protein ID WP_012077229.1
Coordinates 655642..656046 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Y89_RS03090 651300..651588 - 289 Protein_602 DDE-type integrase/transposase/recombinase -
Y89_RS03095 651654..653030 + 1377 WP_076611796.1 IS30-like element ISPa37 family transposase -
Y89_RS03100 653038..653914 - 877 Protein_604 IS3 family transposase -
Y89_RS03105 653979..654843 - 865 Protein_605 site-specific integrase -
Y89_RS03110 655432..655614 + 183 WP_071534354.1 type II toxin-antitoxin system HicA family toxin Toxin
Y89_RS03115 655642..656046 + 405 WP_012077229.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
Y89_RS03120 656089..657060 - 972 WP_012077228.1 hypothetical protein -
Y89_RS03125 657045..657743 - 699 WP_033896049.1 hypothetical protein -
Y89_RS03130 657743..658282 - 540 WP_012074127.1 hypothetical protein -
Y89_RS03135 658279..659199 - 921 WP_012074128.1 hypothetical protein -
Y89_RS03140 659196..659906 - 711 WP_012074129.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 643051..707293 64242


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6702.74 Da        Isoelectric Point: 10.4826

>T108165 WP_071534354.1 NZ_CP030913:655432-655614 [Pseudomonas aeruginosa]
MRSREVIDLLLEDGWYEVAVKGSHHQFKHPSKPGKVTVQHPSSTIPKGTLNNILKQAGLK

Download         Length: 183 bp

>T108165 NZ_CP030913:655432-655614 [Pseudomonas aeruginosa]
ATGCGGAGTAGGGAAGTGATTGATTTGCTGTTGGAGGATGGCTGGTATGAAGTGGCGGTGAAAGGTAGCCACCACCAGTT
CAAACATCCTTCAAAGCCTGGAAAGGTGACGGTTCAACATCCGTCCTCGACGATACCGAAAGGAACCCTGAACAACATAC
TGAAACAAGCCGGCCTCAAGTGA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14670.54 Da        Isoelectric Point: 4.4315

>AT108165 WP_012077229.1 NZ_CP030913:655642-656046 [Pseudomonas aeruginosa]
MKFPVVLHKDPDSDYGVTVPDVPGCFSAGATVSEALANVEEALALHFEGLVTDGEELPQPQDVDAHMKNPDFEGGVWAVV
DFDVTPYLGKAVRFNATLPEHLLQRIDERVKVDKRYQSRSGFLATAAMRELSVA

Download         Length: 405 bp

>AT108165 NZ_CP030913:655642-656046 [Pseudomonas aeruginosa]
ATGAAATTCCCCGTGGTGTTACACAAAGACCCTGATTCTGACTATGGCGTGACTGTGCCCGATGTGCCGGGTTGCTTCTC
TGCCGGCGCTACGGTGTCCGAGGCCCTGGCCAATGTAGAAGAGGCGCTGGCCTTGCACTTCGAAGGCTTGGTTACGGACG
GCGAAGAGCTACCCCAGCCGCAGGACGTTGACGCCCATATGAAGAATCCGGACTTCGAAGGTGGCGTATGGGCGGTGGTT
GACTTCGACGTGACCCCGTACCTGGGCAAGGCCGTACGCTTTAATGCGACGCTCCCGGAACACCTTCTGCAGCGGATCGA
TGAGCGCGTGAAGGTGGATAAGCGCTATCAGTCTCGGTCGGGCTTTCTGGCAACAGCGGCGATGCGCGAACTGTCAGTGG
CGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A6VBH9

Antitoxin

Source ID Structure
AlphaFold DB A0A140SDX7

References