Detailed information of TA system TA107975

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 46824..47236
• Replicon: plasmid unnamed2
• Accession: NZ_CP030780
• Organism: Escherichia albertii strain 05-3106

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A0D8W959
• Locus tag: CYM74_RS23850
• Protein ID: WP_001332444.1
• Coordinates: 46824..46985 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	47051..47236 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CYM74_RS23805	42073..42258	-	186	WP_000797318.1	hypothetical protein	-
CYM74_RS23810	42262..42486	-	225	WP_000786056.1	TraR/DksA C4-type zinc finger protein	-
CYM74_RS23815	42598..43941	-	1344	WP_113623328.1	F-type conjugal transfer pilus assembly protein TraB	-
CYM74_RS23820	43984..44766	-	783	WP_001317493.1	IS21-like element IS100kyp family helper ATPase IstB	-
CYM74_RS23825	44763..45785	-	1023	WP_000255946.1	IS21-like element IS100 family transposase	-
CYM74_RS24350	46208..46586	+	379	Protein_44	hypothetical protein	-
CYM74_RS23850	46824..46985	-	162	WP_001332444.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	47051..47236	-	186	NuclAT_0	-	Antitoxin
-	47051..47236	-	186	NuclAT_0	-	Antitoxin
-	47051..47236	-	186	NuclAT_0	-	Antitoxin
-	47051..47236	-	186	NuclAT_0	-	Antitoxin
CYM74_RS23860	47193..47507	-	315	WP_000872086.1	hypothetical protein	-
CYM74_RS23865	47504..48223	-	720	WP_113623327.1	plasmid SOS inhibition protein A	-
CYM74_RS23870	48220..48654	-	435	WP_000845922.1	conjugation system SOS inhibitor PsiB	-
CYM74_RS23875	48709..50676	-	1968	WP_000117113.1	ParB/RepB/Spo0J family partition protein	-
CYM74_RS23880	50738..50971	-	234	WP_000005968.1	DUF905 domain-containing protein	-
CYM74_RS23885	51028..51555	-	528	WP_000290805.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1 / tet(A) / blaTEM-1B / dfrA15 / ant(3'')-Ia	-	1..80632	80632

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5976.19 Da
• Isoelectric Point: 8.5101

>T107975 WP_001332444.1 NZ_CP030780:c46985-46824 [Escherichia albertii]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 162 bp

>T107975 NZ_CP030780:c46985-46824 [Escherichia albertii]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTAATATTCACACTCCTGACCCG
GAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAGGTTACGGCAAGTTTGGCTTACGAATCCAGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 186 bp

>AT107975 NZ_CP030780:c47236-47051 [Escherichia albertii]
CCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGGATATGAGCTATGATGTG
CCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGCAGAAAGCCCCGT
AGTTAATTTTCATTAACCCACGAGGC

Structures

Toxin

• AlphaFold DB: A0A0D8W959

Antitoxin