Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 5098325..5099156 Replicon chromosome
Accession NZ_CP030768
Organism Escherichia coli strain 2017C-4173W12

Toxin (Protein)


Gene name yeeV Uniprot ID A0A6C9I6Z5
Locus tag DSB71_RS26830 Protein ID WP_001563930.1
Coordinates 5098782..5099156 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag DSB71_RS26825 Protein ID WP_001285585.1
Coordinates 5098325..5098693 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DSB71_RS26790 5095214..5095669 + 456 WP_000581504.1 hypothetical protein -
DSB71_RS26795 5095748..5095981 + 234 WP_001119729.1 DUF905 family protein -
DSB71_RS26805 5096081..5096899 + 819 WP_023910202.1 DUF945 domain-containing protein -
DSB71_RS26810 5096990..5097475 + 486 WP_000214393.1 antirestriction protein -
DSB71_RS26815 5097491..5097967 + 477 WP_023910200.1 RadC family protein -
DSB71_RS26820 5098030..5098251 + 222 WP_000692323.1 DUF987 domain-containing protein -
DSB71_RS26825 5098325..5098693 + 369 WP_001285585.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
DSB71_RS26830 5098782..5099156 + 375 WP_001563930.1 type IV toxin-antitoxin system toxin CbtA Toxin
DSB71_RS26835 5099153..5099347 + 195 WP_000988600.1 hypothetical protein -
DSB71_RS26840 5099360..5099473 + 114 WP_001161660.1 DUF957 domain-containing protein -
DSB71_RS26845 5099762..5099935 - 174 WP_071592185.1 transposase domain-containing protein -
DSB71_RS26850 5099962..5100144 + 183 WP_001016348.1 hypothetical protein -
DSB71_RS26855 5100245..5100574 - 330 WP_000450409.1 DUF496 family protein -
DSB71_RS26860 5100746..5101804 - 1059 WP_001200891.1 FUSC family protein -
DSB71_RS26865 5102002..5102475 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
DSB71_RS26870 5102594..5103760 - 1167 WP_001563931.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13883.97 Da        Isoelectric Point: 7.8522

>T107926 WP_001563930.1 NZ_CP030768:5098782-5099156 [Escherichia coli]
MKTLPVLPGLAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T107926 NZ_CP030768:5098782-5099156 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCTGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT107926 WP_001285585.1 NZ_CP030768:5098325-5098693 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT107926 NZ_CP030768:5098325-5098693 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C9I6Z5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References