Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 779419..780073 Replicon chromosome
Accession NZ_CP030768
Organism Escherichia coli strain 2017C-4173W12

Toxin (Protein)


Gene name cptA Uniprot ID B7N7E0
Locus tag DSB71_RS03740 Protein ID WP_000244769.1
Coordinates 779419..779826 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag DSB71_RS03745 Protein ID WP_000354046.1
Coordinates 779807..780073 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DSB71_RS03720 775376..777109 - 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -
DSB71_RS03725 777115..777825 - 711 WP_000715210.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
DSB71_RS03730 777850..778746 - 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
DSB71_RS03735 778858..779379 + 522 WP_001055874.1 flavodoxin FldB -
DSB71_RS03740 779419..779826 - 408 WP_000244769.1 protein YgfX Toxin
DSB71_RS03745 779807..780073 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
DSB71_RS03750 780316..781296 + 981 WP_000886049.1 tRNA-modifying protein YgfZ -
DSB71_RS03755 781373..782032 - 660 WP_000250274.1 hemolysin III family protein -
DSB71_RS03760 782196..782507 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
DSB71_RS03765 782552..783985 + 1434 WP_001513393.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16062.03 Da        Isoelectric Point: 11.5554

>T107892 WP_000244769.1 NZ_CP030768:c779826-779419 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGK
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T107892 NZ_CP030768:c779826-779419 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGAAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGTGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT107892 WP_000354046.1 NZ_CP030768:c780073-779807 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT107892 NZ_CP030768:c780073-779807 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829J3T1

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References