Detailed information of TA system TA107737

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3486753..3487590
• Replicon: chromosome
• Accession: NZ_CP030331
• Organism: Escherichia coli strain AR_452

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: CSC39_RS18820
• Protein ID: WP_000227784.1
• Coordinates: 3487048..3487590 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: CSC39_RS18815
• Protein ID: WP_001297137.1
• Coordinates: 3486753..3487064 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC39_RS18790	3481773..3482720	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
CSC39_RS18795	3482742..3484733	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
CSC39_RS18800	3484723..3485337	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
CSC39_RS18805	3485337..3485666	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
CSC39_RS18810	3485678..3486568	+	891	WP_000971332.1	heme o synthase	-
CSC39_RS18815	3486753..3487064	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
CSC39_RS18820	3487048..3487590	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
CSC39_RS18825	3487646..3488581	-	936	WP_001297127.1	sel1 repeat family protein	-
CSC39_RS18830	3488989..3490353	+	1365	WP_001000978.1	MFS transporter	-
CSC39_RS18835	3490481..3490972	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
CSC39_RS18840	3491140..3492051	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T107737 WP_000227784.1 NZ_CP030331:3487048-3487590 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T107737 NZ_CP030331:3487048-3487590 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT107737 WP_001297137.1 NZ_CP030331:3486753-3487064 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT107737 NZ_CP030331:3486753-3487064 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
TGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3