Detailed information of TA system TA107712

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 6025158..6025953
• Replicon: chromosome
• Accession: NZ_CP030327
• Organism: Pseudomonas aeruginosa strain AR_458

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CSC44_RS29000
• Protein ID: WP_019486655.1
• Coordinates: 6025774..6025953 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CSC44_RS28995
• Protein ID: WP_021263386.1
• Coordinates: 6025158..6025733 (-)
• Length: 192 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC44_RS28960	6020844..6021023	+	180	WP_015648950.1	Cro/Cl family transcriptional regulator	-
CSC44_RS28965	6021056..6021439	+	384	WP_015648949.1	hypothetical protein	-
CSC44_RS28970	6021607..6022179	+	573	WP_023096627.1	hypothetical protein	-
CSC44_RS28975	6022182..6023192	+	1011	WP_034061664.1	hypothetical protein	-
CSC44_RS28980	6023320..6023733	+	414	WP_034061691.1	hypothetical protein	-
CSC44_RS28985	6023726..6024169	+	444	WP_019396730.1	RusA family crossover junction endodeoxyribonuclease	-
CSC44_RS28990	6024198..6025067	+	870	WP_034061667.1	hypothetical protein	-
CSC44_RS28995	6025158..6025733	-	576	WP_021263386.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CSC44_RS29000	6025774..6025953	-	180	WP_019486655.1	type II toxin-antitoxin system HicA family toxin	Toxin
CSC44_RS29010	6026413..6026748	+	336	WP_003119044.1	phage holin, lambda family	-
CSC44_RS29015	6026745..6027362	+	618	WP_034058608.1	glycoside hydrolase family 19 protein	-
CSC44_RS29020	6027380..6027811	+	432	WP_170958635.1	hypothetical protein	-
CSC44_RS29025	6027908..6028645	+	738	WP_034061674.1	hypothetical protein	-
CSC44_RS29030	6028860..6029405	+	546	WP_031627904.1	terminase small subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	6000308..6049969	49661

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6605.65 Da
• Isoelectric Point: 11.1475

>T107712 WP_019486655.1 NZ_CP030327:c6025953-6025774 [Pseudomonas aeruginosa]
MKFSEFRRWLRAQGVTFEAGKGSHFKVTAPNGKTTTFADHGAKEMPEPTRKAIIKQLGL

Nucleotide

• Length: 180 bp

>T107712 NZ_CP030327:c6025953-6025774 [Pseudomonas aeruginosa]
ATGAAGTTCAGCGAATTCCGACGCTGGCTGAGGGCACAAGGCGTGACCTTCGAAGCTGGCAAGGGCAGTCACTTCAAGGT
GACCGCCCCGAACGGTAAGACAACGACCTTCGCAGATCACGGCGCCAAAGAAATGCCGGAGCCGACACGCAAGGCGATCA
TCAAGCAACTGGGGCTCTGA

Antitoxin

Protein

• Length: 192 a.a.
• Molecular weight: 21294.78 Da
• Isoelectric Point: 8.3556

>AT107712 WP_021263386.1 NZ_CP030327:c6025733-6025158 [Pseudomonas aeruginosa]
MYNFAIRFEQDDSAPGVAVFCRDLPELNSYGDTVEHALSEAVDAIETTLSLYVDQRRSIPVASAPEPEEHVVHLPAVTVA
KIALWNEMMRRGMRKADLCRVLGIAQVQGDRLVDFLHTSKMEQVESALAALEMRLVICAEPVKQPKQCKGAGCIRDRRLV
KARRLLRARLLELTESSGAFTRAPKIKKPGI

Nucleotide

• Length: 576 bp

>AT107712 NZ_CP030327:c6025733-6025158 [Pseudomonas aeruginosa]
ATGTACAACTTCGCAATCCGTTTCGAGCAGGACGACAGCGCTCCTGGCGTTGCCGTTTTCTGTCGAGACCTTCCAGAACT
GAACAGCTATGGAGACACCGTCGAGCACGCGCTCAGCGAGGCCGTTGATGCCATCGAAACCACATTGTCGCTATACGTTG
ATCAACGCCGATCAATTCCAGTGGCTAGCGCTCCAGAGCCAGAAGAGCATGTCGTACATCTTCCGGCTGTGACCGTCGCA
AAGATCGCTCTATGGAATGAGATGATGAGGCGAGGCATGAGAAAGGCAGATCTATGCCGGGTGCTTGGTATCGCCCAAGT
GCAGGGAGATCGTCTTGTCGACTTCTTGCATACATCGAAAATGGAGCAGGTCGAAAGCGCACTGGCCGCACTTGAAATGC
GCCTTGTGATTTGCGCCGAGCCAGTCAAGCAACCGAAGCAGTGCAAGGGCGCAGGGTGTATTCGAGATCGGCGACTCGTT
AAAGCACGCCGCCTGCTGCGCGCCCGGCTCCTTGAACTAACAGAATCTAGCGGCGCGTTCACGCGCGCTCCCAAAATCAA
AAAGCCCGGCATCTGA