Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4016634..4017253 Replicon chromosome
Accession NZ_CP030315
Organism Klebsiella pneumoniae strain 11305

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag DRB03_RS19700 Protein ID WP_002892050.1
Coordinates 4017035..4017253 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag DRB03_RS19695 Protein ID WP_002892066.1
Coordinates 4016634..4017008 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRB03_RS19685 (4011786) 4011786..4012979 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
DRB03_RS19690 (4013002) 4013002..4016148 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
DRB03_RS19695 (4016634) 4016634..4017008 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
DRB03_RS19700 (4017035) 4017035..4017253 + 219 WP_002892050.1 HHA domain-containing protein Toxin
DRB03_RS19705 (4017416) 4017416..4017982 + 567 WP_002892030.1 maltose O-acetyltransferase -
DRB03_RS19710 (4017954) 4017954..4018094 - 141 WP_004147370.1 hypothetical protein -
DRB03_RS19715 (4018115) 4018115..4018585 + 471 WP_020802585.1 YlaC family protein -
DRB03_RS19720 (4018560) 4018560..4020011 - 1452 WP_004183206.1 PLP-dependent aminotransferase family protein -
DRB03_RS19725 (4020112) 4020112..4020810 + 699 WP_023287311.1 GNAT family protein -
DRB03_RS19730 (4020807) 4020807..4020947 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
DRB03_RS19735 (4020947) 4020947..4021210 - 264 WP_032432663.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T107645 WP_002892050.1 NZ_CP030315:4017035-4017253 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T107645 NZ_CP030315:4017035-4017253 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT107645 WP_002892066.1 NZ_CP030315:4016634-4017008 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT107645 NZ_CP030315:4016634-4017008 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References