Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 14767..15292 Replicon plasmid p205880-6
Accession NZ_CP030308
Organism Klebsiella pneumoniae strain 205880

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag DRB04_RS29100 Protein ID WP_013023785.1
Coordinates 14987..15292 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag DRB04_RS29095 Protein ID WP_001568025.1
Coordinates 14767..14985 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRB04_RS29075 (DRB04_30105) 9923..10702 + 780 WP_013023780.1 hypothetical protein -
DRB04_RS29080 (DRB04_30110) 10978..12501 + 1524 WP_017899887.1 hypothetical protein -
DRB04_RS29085 (DRB04_30115) 12535..13662 + 1128 WP_013023782.1 DUF4238 domain-containing protein -
DRB04_RS29090 (DRB04_30120) 13659..13949 + 291 WP_013023783.1 hypothetical protein -
DRB04_RS29095 (DRB04_30140) 14767..14985 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
DRB04_RS29100 (DRB04_30145) 14987..15292 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
DRB04_RS29105 (DRB04_30150) 15461..15856 + 396 WP_017899885.1 hypothetical protein -
DRB04_RS29110 (DRB04_30155) 15883..16197 + 315 WP_053389906.1 hypothetical protein -
DRB04_RS29115 (DRB04_30160) 16208..17224 + 1017 WP_017899884.1 hypothetical protein -
DRB04_RS29120 (DRB04_30165) 17422..18216 + 795 WP_004197635.1 site-specific integrase -
DRB04_RS29125 (DRB04_30170) 18317..19021 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
DRB04_RS29130 (DRB04_30175) 19082..19918 - 837 WP_000480968.1 aminoglycoside O-phosphotransferase APH(6)-Id -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(6)-Id / aph(3'')-Ib / sul2 / tet(A) / aac(3)-IId / dfrA12 / aadA2 / qacE / sul1 / mph(A) - 1..79463 79463


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T107589 WP_013023785.1 NZ_CP030308:14987-15292 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T107589 NZ_CP030308:14987-15292 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT107589 WP_001568025.1 NZ_CP030308:14767-14985 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT107589 NZ_CP030308:14767-14985 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References