Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5397868..5398493 Replicon chromosome
Accession NZ_CP030292
Organism Klebsiella pneumoniae strain 721005

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag DRB00_RS26625 Protein ID WP_002882817.1
Coordinates 5397868..5398251 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag DRB00_RS26630 Protein ID WP_004150355.1
Coordinates 5398251..5398493 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRB00_RS26610 (DRB00_27445) 5395234..5396136 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
DRB00_RS26615 (DRB00_27450) 5396133..5396768 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
DRB00_RS26620 (DRB00_27455) 5396765..5397694 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
DRB00_RS26625 (DRB00_27460) 5397868..5398251 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
DRB00_RS26630 (DRB00_27465) 5398251..5398493 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
DRB00_RS26635 (DRB00_27470) 5398698..5399615 + 918 WP_002882812.1 alpha/beta hydrolase -
DRB00_RS26640 (DRB00_27475) 5399629..5400570 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
DRB00_RS26645 (DRB00_27480) 5400615..5401052 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
DRB00_RS26650 (DRB00_27485) 5401049..5401909 - 861 WP_002882807.1 virulence factor BrkB family protein -
DRB00_RS26655 (DRB00_27490) 5401903..5402502 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T107504 WP_002882817.1 NZ_CP030292:c5398251-5397868 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T107504 NZ_CP030292:c5398251-5397868 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT107504 WP_004150355.1 NZ_CP030292:c5398493-5398251 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT107504 NZ_CP030292:c5398493-5398251 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References