Detailed information of TA system TA107313

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 42952..43577
• Replicon: chromosome
• Accession: NZ_CP030174
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain 11219

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DQB71_RS00220
• Protein ID: WP_021312635.1
• Coordinates: 43194..43577 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: DQB71_RS00215
• Protein ID: WP_004150355.1
• Coordinates: 42952..43194 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DQB71_RS00190	38943..39542	+	600	WP_004151865.1	glucose-1-phosphatase	-
DQB71_RS00195	39536..40396	+	861	WP_002882807.1	virulence factor BrkB family protein	-
DQB71_RS00200	40393..40830	+	438	WP_002882809.1	D-tyrosyl-tRNA(Tyr) deacylase	-
DQB71_RS00205	40875..41816	+	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
DQB71_RS00210	41830..42747	-	918	WP_065889708.1	alpha/beta hydrolase	-
DQB71_RS00215	42952..43194	+	243	WP_004150355.1	ribbon-helix-helix domain-containing protein	Antitoxin
DQB71_RS00220	43194..43577	+	384	WP_021312635.1	type II toxin-antitoxin system VapC family toxin	Toxin
DQB71_RS00225	43751..44680	-	930	WP_004146237.1	formate dehydrogenase accessory protein FdhE	-
DQB71_RS00230	44677..45312	-	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
DQB71_RS00235	45309..46211	-	903	WP_002882822.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14349.56 Da
• Isoelectric Point: 7.3178

>T107313 WP_021312635.1 NZ_CP030174:43194-43577 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVATPYEIHPE

Nucleotide

• Length: 384 bp

>T107313 NZ_CP030174:43194-43577 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGCCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT107313 WP_004150355.1 NZ_CP030174:42952-43194 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT107313 NZ_CP030174:42952-43194 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9