Detailed information of TA system TA107297

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1900796..1901386
• Replicon: chromosome
• Accession: NZ_CP030173
• Organism: Klebsiella variicola strain 13450

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: DQB70_RS09520
• Protein ID: WP_012541133.1
• Coordinates: 1901054..1901386 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: DQB70_RS09515
• Protein ID: WP_016161431.1
• Coordinates: 1900796..1901053 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DQB70_RS09490	1896404..1896979	+	576	WP_012541129.1	hypothetical protein	-
DQB70_RS09495	1897158..1897994	+	837	WP_008804155.1	alpha/beta hydrolase	-
DQB70_RS09500	1898201..1899172	+	972	WP_012541130.1	sensor domain-containing diguanylate cyclase	-
DQB70_RS09505	1899169..1900269	-	1101	WP_022065756.1	hypothetical protein	-
DQB70_RS09515	1900796..1901053	+	258	WP_016161431.1	hypothetical protein	Antitoxin
DQB70_RS09520	1901054..1901386	+	333	WP_012541133.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DQB70_RS09525	1901717..1902817	-	1101	WP_022065434.1	Retron-type reverse transcriptase	-
DQB70_RS09530	1902932..1905178	-	2247	WP_032730759.1	retron Eco8 family effector endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11819.67 Da
• Isoelectric Point: 10.1839

>T107297 WP_012541133.1 NZ_CP030173:1901054-1901386 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNVARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Nucleotide

• Length: 333 bp

>T107297 NZ_CP030173:1901054-1901386 [Klebsiella variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTCGTGGTTCCCGTGACCAGCGGCGGAAACGTTGCACGTA
CAGCAGGCTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCTGACGCCGTTGTCAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTCAGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9450.94 Da
• Isoelectric Point: 4.4915

>AT107297 WP_016161431.1 NZ_CP030173:1900796-1901053 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT107297 NZ_CP030173:1900796-1901053 [Klebsiella variicola]
ATGTACAAAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACAGACAGCGAAGTGGGTATGACTATAGATAATGGCTGCCTGATTATTGAACCGCAGAAACGGCCCCATTATTCGC
TTGAGGAACTGTTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA