Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 8433..8955 Replicon plasmid pJSMCR1_4
Accession NZ_CP030156
Organism Escherichia coli strain JSMCR1

Toxin (Protein)


Gene name relE Uniprot ID U9YLU1
Locus tag JSMCR1_RS25450 Protein ID WP_000638823.1
Coordinates 8433..8714 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID U9Y7J1
Locus tag JSMCR1_RS25455 Protein ID WP_000121741.1
Coordinates 8704..8955 (-) Length 84 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSMCR1_RS25415 (JSMCR1_p563) 4582..4902 - 321 WP_000362080.1 VirB3 family type IV secretion system protein -
JSMCR1_RS25420 (JSMCR1_p564) 4973..5263 - 291 WP_000865479.1 conjugal transfer protein -
JSMCR1_RS25425 (JSMCR1_p565) 5263..5847 - 585 WP_001177113.1 lytic transglycosylase domain-containing protein -
JSMCR1_RS25430 (JSMCR1_p566) 5868..6266 - 399 WP_001153665.1 hypothetical protein -
JSMCR1_RS25435 (JSMCR1_p567) 6385..6822 - 438 WP_000539665.1 type IV pilus biogenesis protein PilM -
JSMCR1_RS25440 (JSMCR1_p568) 6828..8063 - 1236 WP_015059538.1 TcpQ domain-containing protein -
JSMCR1_RS25445 (JSMCR1_p569) 8066..8365 - 300 WP_000835764.1 TrbM/KikA/MpfK family conjugal transfer protein -
JSMCR1_RS25450 (JSMCR1_p570) 8433..8714 - 282 WP_000638823.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JSMCR1_RS25455 (JSMCR1_p571) 8704..8955 - 252 WP_000121741.1 hypothetical protein Antitoxin
JSMCR1_RS25460 (JSMCR1_p572) 9055..9690 - 636 WP_015059536.1 hypothetical protein -
JSMCR1_RS25465 (JSMCR1_p573) 9763..10050 - 288 WP_001032611.1 EexN family lipoprotein -
JSMCR1_RS25470 (JSMCR1_p574) 10063..10317 - 255 WP_001043555.1 EexN family lipoprotein -
JSMCR1_RS25475 (JSMCR1_p575) 10319..10960 - 642 WP_001425343.1 type IV secretion system protein -
JSMCR1_RS25480 (JSMCR1_p576) 10966..11961 - 996 WP_001028543.1 type IV secretion system protein -
JSMCR1_RS25485 (JSMCR1_p577) 11965..12222 - 258 WP_000739144.1 hypothetical protein -
JSMCR1_RS25490 (JSMCR1_p578) 12219..12521 - 303 WP_001360345.1 hypothetical protein -
JSMCR1_RS25495 (JSMCR1_p579) 12502..12759 - 258 WP_001542015.1 hypothetical protein -
JSMCR1_RS25500 (JSMCR1_p580) 12792..13238 - 447 WP_001243165.1 hypothetical protein -
JSMCR1_RS25505 (JSMCR1_p581) 13249..13419 - 171 WP_000550720.1 hypothetical protein -
JSMCR1_RS25510 (JSMCR1_p582) 13423..13866 - 444 WP_000964330.1 NfeD family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-1.1 - 1..61828 61828


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-87)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 94 a.a.        Molecular weight: 10945.79 Da        Isoelectric Point: 10.4997

>T107252 WP_000638823.1 NZ_CP030156:c8714-8433 [Escherichia coli]
MIYELAFDHRALKEWQKLGHTVREQFKKKLSERLINPRVPAAKLHGHTDRYKIKLRASGYRLVYQVIDEKVVLLVISVGK
REGSDVYHMADTR

Download         Length: 282 bp

>T107252 NZ_CP030156:c8714-8433 [Escherichia coli]
ATGATTTATGAACTGGCATTTGACCACAGAGCACTGAAGGAATGGCAAAAACTCGGTCACACTGTTCGTGAGCAATTCAA
AAAGAAACTGTCAGAACGCCTGATAAATCCACGCGTCCCCGCAGCCAAGCTACATGGTCATACTGACCGTTACAAAATTA
AGCTTCGCGCATCCGGTTACAGGCTCGTTTATCAGGTAATTGATGAGAAGGTCGTTTTGCTTGTGATTTCTGTGGGAAAA
AGAGAAGGCAGCGACGTCTATCACATGGCAGATACCCGCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9223.38 Da        Isoelectric Point: 3.9823

>AT107252 WP_000121741.1 NZ_CP030156:c8955-8704 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPELYAYYRELAEDAELNAIADERMKNPDIVRVNL
DDL

Download         Length: 252 bp

>AT107252 NZ_CP030156:c8955-8704 [Escherichia coli]
ATGAGCTACCAAATTCTGACTACGACAGCAGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACTGTAGCCGAAGG
AGAAGGGGATGCCGTCGCAATCCTGAACCGAAATGAACCAGCGTTCTACTGTGTCCCACCAGAACTTTATGCCTACTACC
GGGAACTCGCTGAAGACGCTGAACTCAACGCAATTGCTGATGAAAGAATGAAAAATCCAGACATTGTAAGGGTTAACCTG
GATGATTTATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB U9YLU1


Antitoxin

Source ID Structure
AlphaFold DB U9Y7J1

References