Detailed information of TA system TA107133

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4406524..4407356
• Replicon: chromosome
• Accession: NZ_CP030111
• Organism: Escherichia coli strain MCJCHV-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: DPQ29_RS22860
• Protein ID: WP_000854753.1
• Coordinates: 4406982..4407356 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NQ68
• Locus tag: DPQ29_RS22855
• Protein ID: WP_001540478.1
• Coordinates: 4406524..4406892 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DPQ29_RS22820	4402357..4403037	+	681	WP_001282919.1	WYL domain-containing protein	-
DPQ29_RS22825	4403185..4403862	+	678	WP_001097301.1	hypothetical protein	-
DPQ29_RS22830	4403868..4404101	+	234	WP_001278283.1	DUF905 family protein	-
DPQ29_RS22835	4404191..4405009	+	819	WP_001773857.1	DUF945 domain-containing protein	-
DPQ29_RS22840	4405100..4405585	+	486	WP_000214398.1	antirestriction protein	-
DPQ29_RS22845	4405601..4406077	+	477	WP_001186786.1	RadC family protein	-
DPQ29_RS22850	4406140..4406361	+	222	WP_000692311.1	DUF987 domain-containing protein	-
DPQ29_RS22855	4406524..4406892	+	369	WP_001540478.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DPQ29_RS22860	4406982..4407356	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
DPQ29_RS22865	4407353..4407841	+	489	WP_000777545.1	hypothetical protein	-
DPQ29_RS22870	4407853..4408050	+	198	WP_000839281.1	DUF957 domain-containing protein	-
DPQ29_RS22875	4408147..4408716	+	570	WP_001560692.1	DUF4942 domain-containing protein	-
DPQ29_RS22885	4409465..4411003	+	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4362498..4418816	56318

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T107133 WP_000854753.1 NZ_CP030111:4406982-4407356 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T107133 NZ_CP030111:4406982-4407356 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13652.53 Da
• Isoelectric Point: 7.8398

>AT107133 WP_001540478.1 NZ_CP030111:4406524-4406892 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT107133 NZ_CP030111:4406524-4406892 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: S1NQ68