Detailed information of TA system TA107128

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-timR/SymE(toxin)
• Location: 4111112..4111524
• Replicon: chromosome
• Accession: NZ_CP030111
• Organism: Escherichia coli strain MCJCHV-1

Toxin (Protein)

• Gene name: symE
• Uniprot ID: S1NWQ7
• Locus tag: DPQ29_RS21200
• Protein ID: WP_000132614.1
• Coordinates: 4111183..4111524 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	timR
Locus tag	-
Coordinates	4111112..4111188 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DPQ29_RS21190	4107724..4109193	+	1470	WP_001387312.1	type I restriction-modification system subunit M	-
DPQ29_RS21195	4109193..4110962	+	1770	WP_001513535.1	restriction endonuclease subunit S	-
-	4111112..4111188	-	77	NuclAT_8	-	Antitoxin
-	4111112..4111188	-	77	NuclAT_8	-	Antitoxin
-	4111112..4111188	-	77	NuclAT_8	-	Antitoxin
-	4111112..4111188	-	77	NuclAT_8	-	Antitoxin
-	4111112..4111188	-	77	NuclAT_9	-	Antitoxin
-	4111112..4111188	-	77	NuclAT_9	-	Antitoxin
-	4111112..4111188	-	77	NuclAT_9	-	Antitoxin
-	4111112..4111188	-	77	NuclAT_9	-	Antitoxin
DPQ29_RS21200	4111183..4111524	+	342	WP_000132614.1	endoribonuclease SymE	Toxin
DPQ29_RS21205	4111571..4113727	-	2157	WP_001513533.1	DUF262 domain-containing protein	-
DPQ29_RS21210	4113762..4113842	-	81	WP_020233658.1	hypothetical protein	-
DPQ29_RS21215	4114070..4114990	-	921	WP_001513532.1	Rpn family recombination-promoting nuclease/putative transposase	-
DPQ29_RS21220	4115175..4116455	+	1281	WP_001338077.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	cheD	4095500..4111524	16024

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12266.07 Da
• Isoelectric Point: 8.4982

>T107128 WP_000132614.1 NZ_CP030111:4111183-4111524 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T107128 NZ_CP030111:4111183-4111524 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACTGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT107128 NZ_CP030111:c4111188-4111112 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1NWQ7

Antitoxin