Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 179450..180186 Replicon plasmid pDA12090.1
Accession NZ_CP030071
Organism Klebsiella pneumoniae strain DA12090

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag DL426_RS01060 Protein ID WP_003026803.1
Coordinates 179704..180186 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag DL426_RS01055 Protein ID WP_003026799.1
Coordinates 179450..179716 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DL426_RS01010 175512..175874 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
DL426_RS01015 175924..176274 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
DL426_RS01020 176632..176901 + 270 WP_004152102.1 hypothetical protein -
DL426_RS01025 176889..177464 + 576 WP_004152103.1 hypothetical protein -
DL426_RS01030 177495..177989 + 495 WP_004152104.1 DNA-binding protein -
DL426_RS01035 178033..178401 + 369 WP_004152105.1 hypothetical protein -
DL426_RS01040 178435..178638 + 204 WP_004152106.1 hemolysin expression modulator Hha -
DL426_RS01045 178687..178944 + 258 WP_004152107.1 hypothetical protein -
DL426_RS01050 179020..179274 + 255 WP_004152108.1 hypothetical protein -
DL426_RS01055 179450..179716 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
DL426_RS01060 179704..180186 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
DL426_RS01065 180345..181599 - 1255 Protein_195 IS3 family transposase -
DL426_RS01070 181687..182649 - 963 WP_004152113.1 M48 family metalloprotease -
DL426_RS01075 182636..183124 - 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -
DL426_RS01090 183622..183819 - 198 WP_004152115.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..185511 185511


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T106990 WP_003026803.1 NZ_CP030071:179704-180186 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T106990 NZ_CP030071:179704-180186 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT106990 WP_003026799.1 NZ_CP030071:179450-179716 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT106990 NZ_CP030071:179450-179716 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References