Detailed information of TA system TA106962

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 545861..546453
• Replicon: plasmid unnamed
• Accession: NZ_CP030056
• Organism: Bradyrhizobium sp. CCBAU 53340

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: XH89_RS39160
• Protein ID: WP_128929956.1
• Coordinates: 545861..546049 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: XH89_RS39165
• Protein ID: WP_128929955.1
• Coordinates: 546055..546453 (+)
• Length: 133 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XH89_RS39130	541779..542108	-	330	WP_128930197.1	EAL domain-containing protein	-
XH89_RS39135	542177..542551	+	375	WP_188637391.1	hypothetical protein	-
XH89_RS39140	542728..543201	+	474	WP_128929538.1	transposase	-
XH89_RS39145	543198..543552	+	355	Protein_508	IS66 family insertion sequence element accessory protein TnpB	-
XH89_RS39150	543603..545162	+	1560	WP_128929539.1	IS66 family transposase	-
XH89_RS39155	545195..545785	+	591	WP_128930143.1	UPF0149 family protein	-
XH89_RS39160	545861..546049	+	189	WP_128929956.1	type II toxin-antitoxin system HicA family toxin	Toxin
XH89_RS39165	546055..546453	+	399	WP_128929955.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
XH89_RS39170	547107..548303	-	1197	Protein_513	Fic family protein	-
XH89_RS39175	548781..549359	+	579	WP_128929954.1	helix-turn-helix domain-containing protein	-
XH89_RS39180	549352..550575	+	1224	WP_128929953.1	type II toxin-antitoxin system HipA family toxin	-
XH89_RS39185	550854..551174	-	321	WP_128929952.1	ferredoxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd	1..979292	979292

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7077.32 Da
• Isoelectric Point: 11.2092

>T106962 WP_128929956.1 NZ_CP030056:545861-546049 [Bradyrhizobium sp. CCBAU 53340]
MKSREIISILQGDGWFEVGRKGSHVQFKHPFKKGRVIVPHPERDVPLGTLKSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T106962 NZ_CP030056:545861-546049 [Bradyrhizobium sp. CCBAU 53340]
ATGAAAAGCCGGGAAATTATCTCGATCCTTCAAGGGGATGGATGGTTTGAGGTGGGCCGGAAAGGCAGCCATGTGCAGTT
CAAACATCCATTCAAGAAGGGTCGCGTAATTGTTCCGCACCCCGAACGGGATGTCCCACTCGGGACACTGAAGAGTATTG
AAAAGCAGTCAGGTCTCAAACTGAGGTAA

Antitoxin

Protein

• Length: 133 a.a.
• Molecular weight: 14163.96 Da
• Isoelectric Point: 4.3695

>AT106962 WP_128929955.1 NZ_CP030056:546055-546453 [Bradyrhizobium sp. CCBAU 53340]
MRNYIGLIHKDADSDFGVSFPDFPGVITAGKSLDDARAMAEEALTLHIEGLAEDGEAIPEPSTLEDVMADPDHRTGVAIL
VSVKPEQPKAIRVNVTLPEDVLNQIDKYAEAHGFTRSGLLTQAAKRLIGEAA

Nucleotide

• Length: 399 bp

>AT106962 NZ_CP030056:546055-546453 [Bradyrhizobium sp. CCBAU 53340]
ATGCGTAACTATATTGGTCTTATCCATAAGGATGCCGACAGCGATTTCGGCGTCTCATTTCCAGACTTCCCCGGAGTGAT
CACGGCAGGCAAAAGCCTTGATGACGCTCGTGCTATGGCAGAGGAAGCCCTCACCCTTCATATCGAGGGTCTTGCTGAAG
ATGGAGAAGCCATTCCGGAGCCCTCTACTCTAGAGGATGTTATGGCAGATCCTGATCATCGAACTGGGGTAGCTATTTTG
GTGTCGGTCAAACCCGAACAACCAAAGGCTATACGTGTGAATGTCACCCTTCCTGAGGACGTTCTGAATCAGATCGACAA
ATATGCTGAAGCGCACGGCTTTACGAGATCGGGCCTCCTAACTCAGGCCGCGAAGAGACTCATAGGAGAAGCAGCCTAA