Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2806917..2807555 Replicon chromosome
Accession NZ_CP029981
Organism Escherichia coli strain 99-3165

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag B4O06_RS15710 Protein ID WP_000813794.1
Coordinates 2807379..2807555 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag B4O06_RS15705 Protein ID WP_001270286.1
Coordinates 2806917..2807333 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B4O06_RS15685 2802069..2803010 - 942 WP_001251313.1 ABC transporter permease -
B4O06_RS15690 2803011..2804024 - 1014 WP_000220399.1 ABC transporter ATP-binding protein -
B4O06_RS15695 2804042..2805187 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
B4O06_RS15700 2805432..2806838 - 1407 WP_000760586.1 PLP-dependent aminotransferase family protein -
B4O06_RS15705 2806917..2807333 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
B4O06_RS15710 2807379..2807555 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
B4O06_RS15715 2807777..2808007 + 231 WP_000494244.1 YncJ family protein -
B4O06_RS15720 2808099..2810060 - 1962 WP_001355676.1 23S rRNA 5-hydroxycytidine C2501 synthase -
B4O06_RS15725 2810133..2810669 - 537 WP_000429133.1 DNA-binding transcriptional regulator SutR -
B4O06_RS15730 2810722..2811936 + 1215 WP_071597387.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T106819 WP_000813794.1 NZ_CP029981:c2807555-2807379 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T106819 NZ_CP029981:c2807555-2807379 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT106819 WP_001270286.1 NZ_CP029981:c2807333-2806917 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT106819 NZ_CP029981:c2807333-2806917 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References